CircRNA TADA2A relieves idiopathic pulmonary fibrosis by inhibiting proliferation and activation of fibroblasts.

Cell Death & Disease
Juan LiWei Zhao

Abstract

The excessive activation and proliferation of lung fibroblasts are responsible for the abundant deposition of extracellular matrix (ECM) in idiopathic pulmonary fibrosis (IPF), while its specific mechanism is still unknown. This study focuses on the role of circRNA (circ) TADA2A in functional abnormalities of lung fibroblasts and aims to elaborate its regulatory mechanism. In the present study, circTADA2A was downregulated in both IPF primary human lung fibroblasts and human IPF fibroblastic cell lines. Functionally, the overexpression of circTADA2A repressed the activation and proliferation of normal human fibroblastic cell line induced by several fibrogenic growth factors. Using fluorescence in situ hybridization (FISH), luciferase reporter assays, and RNA pull-down, circTADA2A was confirmed to function as sponges of miR-526b and miR-203, thus releasing the expression of Caveolin (Cav)-1 and Cav2. The overexpression of circTADA2A suppressed lung-fibroblasts activation via Cav1 and reduced lung-fibroblasts proliferation via Cav2. In vivo experiments also confirmed that the overexpression of circTADA2A decreased fibrogenic responses induced by bleomycin in lung-fibrosis mice. Collectively, circTADA2A repressed lung-fibroblasts ...Continue Reading

References

Nov 5, 2003·The Journal of Pathology·Eric S WhiteVictor J Thannickal
Dec 21, 2006·The Journal of Experimental Medicine·Xiao Mei WangAugustine M K Choi
Jun 11, 2010·Annual Review of Biochemistry·Marc Robert FabianWitold Filipowicz
Dec 23, 2011·The American Journal of Pathology·Shanzhong YangGang Liu
Apr 16, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·David GvaramiaVincent Everts
Apr 27, 2013·Science·Jeremy E Wilusz, Phillip A Sharp
Sep 27, 2013·Cell Cycle·Cecilia J G de AlmeidaMichael P Lisanti
May 27, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Heidi N FridolfssonHemal H Patel
Oct 30, 2016·The Journal of Pathology·Janette K BurgessGunilla Westergren-Thorsson
Oct 28, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Saeid GhavamiAndrew J Halayko
Dec 9, 2017·EMBO Molecular Medicine·Hamza M Al-TamariSoni S Pullamsetti
Apr 27, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·David W WatersDarryl A Knight
Apr 10, 2019·American Journal of Respiratory and Critical Care Medicine·Grégoire SavaryNicolas Pottier
Jul 19, 2019·Frontiers in Genetics·Yujiao ZhuLijun Wang

❮ Previous
Next ❯

Citations

Feb 22, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xiaoniu DaiJie Chao

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
enzymatic dissociation
transfection
ELISA
electrophoresis
Assay
pull-down
FCS

Software Mentioned

GraphPad

Related Concepts

Related Feeds

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.