CircRNA_0005075 suppresses carcinogenesis via regulating miR-431/p53/epithelial-mesenchymal transition axis in gastric cancer.

Cell Biochemistry and Function
Jiaming WuYuping Peng

Abstract

This study was aimed to explore the expression and biological function of circRNA_0005075 in gastric cancer (GC) progression and its underlying mechanism. First, the expression level of circRNA_0005075 and microRNA-431 (miR-431) in GC tissues were detected with the quantitative real-time polymerase chain reaction. In addition, after down-regulated the circRNA_0005075 expression by plasmid transfection in GC cells, the Cell Counting Kit-8 (CCK-8), EDU, transwell assay were conducted to evaluate the function of circRNA_0005075 or miR-431 on cell proliferation, metastasis in vitro. Moreover, p53 and Epithelial-mesenchymal transition (EMT) pathway related proteins were also measured with western blotting. Then, our data revealed that CircRNA_0005075 was found to be significantly up-regulated in GC tissues as well as GC cell lines, and the GC patients with higher CircRNA_0005075 expression were more likely to have poor outcomes. Down-regulation of CircRNA_0005075 could significantly suppress the GC cell proliferation and cell metastasis ability, while the addition of miR-431 inhibitors could counteract this effect. Importantly, we discovered that the silencing of circRNA_0005075 could weaken the micro-RNA sponge function for miR-431...Continue Reading

References

Mar 29, 2006·The Journal of Cell Biology·Jonathan M LeeErik W Thompson
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Mar 1, 2013·Nature·Sebastian MemczakNikolaus Rajewsky
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Jul 2, 2016·Cell·M Angela NietoJean Paul Thiery
Jan 13, 2018·Nature Reviews. Cancer·Thomas BrabletzRobert A Weinberg
May 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ming-Fang LiJun Li
Dec 20, 2018·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Chao HanChanghong Lian
Dec 26, 2018·Cell·Sung-Hwan MoonCarol Prives
Feb 6, 2019·Molecular Oncology·Lorena VerduciGiovanni Blandino
May 6, 2019·Journal of Cellular Physiology·Xiangyu YangKun Gao
Jun 10, 2019·DNA Repair·Amir MohammadzadehMaryam Majidinia
Jul 10, 2019·International Journal of Radiation Biology·Ali SakMartin Stuschke

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