Circular RNA-9119 protects IL-1β-treated chondrocytes from apoptosis in an osteoarthritis cell model by intercepting the microRNA-26a/PTEN axis

Life Sciences
Changjian ChenBaowen Li

Abstract

Osteoarthritis (OA) is a common degenerative joint disease characterized by cartilage degeneration and joint inflammation. As its pathogenesis remains unclear, there are no effective treatments established. Circular RNA (circRNA), microRNA (miRNA), and other noncoding RNAs participate in OA development; however, the effects and mechanisms of circRNA and miRNA in OA remain unknown. Cartilage miRNA was examined in patients with and without OA. CircRNA-9119 and phosphatase and tensin homolog (PTEN) expression decreased in OA-affected cartilage and interleukin (IL)-1β-induced chondrocytes, and miR-26a expression significantly decreased in normal cells and tissues. CircRNA-9119 overexpression restored chondrocyte growth, whereas IL-1β treatment impaired chondrocyte growth. Annexin V-FITC & PI flow cytometry and Bcl-2/Bax ratio measurement indicated that the apoptosis of IL-1β-treated articular chondrocytes was decreased by circRNA-9119 upregulation. Bioinformatic prediction and the dual-luciferase reporter assay indicated that circRNA-9119 served as a miR-26a sponge and that miR-26a targeted the 3'-UTR of PTEN. Transfection of chondrocytes with a circRNA-9119-overexpressing vector revealed downregulation of miR-26a expression. Furth...Continue Reading

Citations

May 3, 2021·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Y ChenY Zhang
May 25, 2021·Molecular Therapy. Nucleic Acids·Xingjia MaoChuan Xiang
Jun 10, 2021·The Journal of International Medical Research·Hao ChenXiaoyong Qiu
Jun 15, 2021·Frontiers in Molecular Biosciences·Jieqing YuJing Ye
Jun 19, 2021·Journal of Orthopaedic Surgery and Research·Yongqiang LiuXuefeng Gao
Oct 1, 2021·Nature Reviews. Rheumatology·Shabana A AliMohit Kapoor
Dec 11, 2021·Frontiers in Pharmacology·Jinxia DengBo Wei
Jan 28, 2022·Molecular Genetics and Genomics : MGG·Bimaljeet KourProsenjit Paul

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis