Circular RNA-9119 suppresses in ovarian cancer cell viability via targeting the microRNA-21-5p-PTEN-Akt pathway.

Aging
Jianming GongYingqiao Zhou

Abstract

We aimed to assess the regulatory role of circular RNA (circRNA)-9119 (circ9119) in ovarian cancer (OC) cell viability. The expression of circ9119 was clearly reduced in OC tissues and cell lines, whereas the microRNA-21-5p (miR-21) levels were elevated compared with those in normal healthy control tissues and immortalized fallopian epithelial cell line FTE187. Further, circ9119 was overexpressed, causing a notable decrease in the viability and proliferation of OC cells and an increase in apoptosis. Further study showed that circ9119 upregulation resulted in a decrease in miR-21 levels. Bioinformatics forecasting (starBase and TargetScan) and dual luciferase reporter assay demonstrated that circ9119 acts as an miR-21 sponge. Recovery of miR-21 expression in circ9119-overexpressing OC cells showed that miR-21 exhibited the opposite effect on circ9119; moreover, its recovery could suppress the effects of circ9119 overexpression, recover cell proliferation, and reduce apoptosis. Furthermore, miR-21 was found to target phosphatase and tensin homologue (PTEN) 3' untranslated region. PTEN protein and mRNA expression was reduced in OC tissues and cells, whereas it was increased on transfection with an miR-21 inhibitor. Thus, circ9119 ...Continue Reading

References

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Citations

Apr 17, 2021·Essays in Biochemistry·Huiwen Yan, Pengcheng Bu
Jul 15, 2021·Reproductive Biology and Endocrinology : RB&E·Ricardo Josué Acuña-GonzálezHéctor Flores-Herrera
Sep 25, 2021·Cellular & Molecular Biology Letters·Zahra ForuzandehMohammad Reza Alivand
Oct 29, 2021·Environmental Science and Pollution Research International·Hui WangHeng Wang

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Datasets Mentioned

BETA
FTE187

Methods Mentioned

BETA
transfection
flow cytometry
xenograft
PCR

Software Mentioned

FlowJo

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