Circular RNA circ-CSPP1 regulates CCNE2 to facilitate hepatocellular carcinoma cell growth via sponging miR-577

Cancer Cell International
Qian SunLianfeng Zhang

Abstract

Circ-centro-some/spindle pole-associated protein (CSPP1) has been confirmed to be characterized in diverse human malignancies and its ectopic expression may regulate tumor progression and development. However, in hepatocellular carcinoma (HCC), its biological role, clinical significance and molecular mechanism are still unclear. Circ-CSPP1 expression and its prognostic values in HCC tissues were detected by qRT-PCR or in situ hybridization (ISH), and enriched by using Rnase R. The functional experiments (Circ-CSPP1 was overexpressed or knocked down) were performed in HCC cells. The HCC cell growth was analyzed by CCK-8 assay, transwell, wound healing and colony formation assays. The interation between circ-CSPP1 and miR-577/miR-577 and cyclin E2 (CCNE2) were determined by dual luciferase assay or RNA binding protein immunoprecipitation (RIP) assay. The RNA fluorescence in situ hybridization (FISH) assay was used to detect the subcellular distribution. Finally, an in vivo nude mouse tumor model was constructed. In HCC patients and cells, circ-CSPP1 was aberrantly expressed, and its upregulation predicted poor prognosis, and closely correlated with tumor size and TNM stage. Circ-CSPP1 resisted RnaseR digestion, indicating it is a...Continue Reading

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Citations

Feb 6, 2021·Journal of Hepatocellular Carcinoma·Abdullah ElyPatrick Arbuthnot
Aug 12, 2021·Cancer Management and Research·Qianggui HeShuibing Cheng

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Datasets Mentioned

BETA
GSE78520
GSE94508
GPL19978
GSE97332

Methods Mentioned

BETA
transfection
PCR
flow cytometry
immunoprecipitation
chip
xenograft
RIP

Software Mentioned

Circular RNA Interactome
SPSS
GEO2R
CellQuest
Starbase
GraphPad Prism
Image J
circbank

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