Circular RNA circ_0000950 promotes neuron apoptosis, suppresses neurite outgrowth and elevates inflammatory cytokines levels via directly sponging miR-103 in Alzheimer's disease

Cell Cycle
Hui YangXuling Li

Abstract

This study aimed to investigate the effect of circ_0000950/miR-103 network on regulating neuron apoptosis, neurite outgrowth and inflammation in Alzheimer's disease (AD). Cellular AD model of rat pheochromocytoma cell line PC12 cells and cellular AD model of rat cerebral cortex neurons were constructed, and the effect of circ_0000950 on apoptosis, neurite outgrowth and inflammation in both cellular AD models was determined through upregulation and knockdown of circ_0000950 expression by transfection. Compensation experiments and luciferase assay were further performed to validate the sponging effect of circ_0000950 on miR-103 as well as the mechanisms of circ_0000950/miR-103 on regulating apoptosis, neurite outgrowth and inflammation in both cellular AD models. Circ_0000950 reduced miR-103 expression and increased prostaglandin-endoperoxide synthase 2 (PTGS2) expression in both two cellular AD models. And circ_0000950 overexpression promoted neuron apoptosis, suppressed neurite outgrowth and elevated IL-1β, IL-6 and TNF-α levels compared with overexpression control, whereas circ_0000950 knockdown inhibited neuron apoptosis, enhanced neurite outgrowth and lowered IL-1β, IL-6 and TNF-α levels compared with shRNA control in both t...Continue Reading

References

Jun 24, 2008·Nature Reviews. Neuroscience·Jürgen Götz, Lars M Ittner
Mar 5, 2011·Lancet·Clive BallardEmma Jones
Mar 20, 2012·Cell·Yadong Huang, Lennart Mucke
Sep 13, 2012·Frontiers in Genetics·Walter J Lukiw
Mar 1, 2013·Nature·Thomas B HansenJørgen Kjems
Jun 12, 2013·Lancet·Kit Yee ChanUNKNOWN Global Health Epidemiology Reference Group (GHERG)
Sep 13, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sabrina AbsalonAnna M Krichevsky
Jan 16, 2014·Frontiers in Genetics·Walter J Lukiw
Jul 30, 2014·Genome Biology·Junjie U GuoDavid P Bartel
Sep 23, 2014·Molecular Cell·Reut Ashwal-FlussSebastian Kadener
Sep 18, 2015·Circulation Research·Jes-Niels BoeckelStefanie Dimmeler
Feb 13, 2016·Cell·Bart De Strooper, Eric Karran
Feb 28, 2016·Lancet·Philip ScheltensWiesje Maria Van der Flier
Aug 20, 2016·Nature Communications·Lesca M HoldtDaniel Teupser
Nov 26, 2016·Cell Death and Differentiation·William W DuBurton B Yang
Jan 29, 2017·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Cassia Overk, Eliezer Masliah
Apr 5, 2017·Frontiers in Neurology·Rose Ann Huynh, Chandra Mohan
May 10, 2017·Experimental Biology and Medicine·Kuei-Yang HsiaoShaw-Jenq Tsai
Apr 17, 2018·Bulletin of Experimental Biology and Medicine·E A BelousovaN E Kushlinskii
Jun 10, 2018·Clinical Biochemistry·Peter D Fransquet, Joanne Ryan

❮ Previous
Next ❯

Citations

Sep 22, 2020·Translational Neurodegeneration·Yuan ZhangLei Zhang
Dec 20, 2020·International Journal of Molecular Sciences·Leticia Moreno-GarcíaRosario Osta
Nov 13, 2020·Frontiers in Neurology·Xuewen XiaoBin Jiao
Apr 6, 2021·Frontiers in Molecular Biosciences·Meng-Lan LiZi-Bing Jin
Jul 30, 2021·Frontiers in Aging Neuroscience·Meng Zhang, Zhigang Bian
Jul 20, 2021·Neurochemistry International·Suresh L MehtaRaghu Vemuganti
Jul 27, 2021·Frontiers in Molecular Neuroscience·Yue QiJun Wan
Aug 21, 2021·Journal of Molecular Neuroscience : MN·Soudeh Ghafouri-FardMohammad Samadian

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
X-ray
transfection

Software Mentioned

Presage
SPSS
GraphPad

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis