Circular RNA circANKRD36 regulates Casz1 by targeting miR-599 to prevent osteoarthritis chondrocyte apoptosis and inflammation.

Journal of Cellular and Molecular Medicine
Jian-Lin ZhouQiong-Jie Hu

Abstract

Osteoarthritis (OA) is an ageing-related disease characterized by articular cartilage degradation and joint inflammation. circRNA has been known to involve in the regulation of multiple inflammatory diseases including OA. However, the mechanism underlying how circRNA regulates OA remains to be elucidated. Here, we report circANKRD36 prevents OA chondrocyte apoptosis and inflammation by targeting miR-599, which specifically degrades Casz1. We performed circRNA sequencing in normal and OA tissues and found the expression of circANKRD36 is decreased in OA tissues. circANKRD36 is also reduced in IL-1β-treated human chondrocytes. FACS analysis and Western blot showed that the knockdown of circANKRD36 promotes the apoptosis and inflammation of chondrocytes in IL-1β stress. We then found miR-599 to be the target of circANKRD36 and correlate well with circANKRD36 both in vitro and in vivo. By database analysis and luciferase assay, Casz1 was found to be the direct target of miR-599. Casz1 helps to prevent apoptosis and inflammation of chondrocytes in response to IL-1β. In conclusion, our results proved circANKRD36 sponge miR-599 to up-regulate the expression of Casz1 and thus prevent apoptosis and inflammation in OA.

References

Mar 1, 2013·Nature·Thomas B HansenJørgen Kjems
Mar 7, 2013·The EMBO Journal·Matthias W Hentze, Thomas Preiss
Jan 17, 2014·Nature·Yvonne TayPier Paolo Pandolfi
Apr 1, 2014·Cell·Thomas R Cech, Joan A Steitz
Oct 1, 2014·Nature Reviews. Rheumatology·Ru Liu-Bryan, Robert Terkeltaub
Nov 19, 2015·Osteoarthritis and Cartilage·Mary Nugent
Jul 15, 2016·Nature Reviews. Rheumatology·Rita VicenteChristian Jorgensen
Oct 14, 2016·Nature Reviews. Disease Primers·Johanne Martel-PelletierJean-Pierre Pelletier
Jan 10, 2018·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xinhua HuXimei Wu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
flow cytometry
Assay
RIP
FACS
ELISA
immunoprecipitation
Flow

Software Mentioned

FlowJo
RegRNA
Tree Star

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis