Circular RNA circBACH2 plays a role in papillary thyroid carcinoma by sponging miR-139-5p and regulating LMO4 expression

Cell Death & Disease
Xiaoyan CaiJun Li

Abstract

Circular RNAs (circRNAs) are a class of non-coding RNAs that are broadly expressed in various biological cells and function in regulating gene expression. They are structurally stable and tissue-specific. However, the function of human circRNAs and the role of circRNAs in papillary thyroid carcinoma (PTC) remain to be determined. Herein, the function of circRNA circBACH2 was investigated in human PTC cells. First, we detected the expression of circBACH2 in PTC tissues and PTC cell lines by RT-PCR. FISH was used to confirm the subcellular localization of circBACH2. A luciferase reporter assay and AGO2-RIP was used to confirm the relationship between circBACH2 and miR-139-5p. PTC cells were stably transfected with siRNA against circBACH2 and cell proliferation, migration and invasion were detected to evaluate the effect of circBACH2 in PTC, while tumorigenesis was assayed in nude mice. We found that circBACH2 was highly expressed in PTC tissues and PTC cell lines. Mechanistically, we confirmed that circBACH2 could directly bind to miR-139-5p and relieve suppression of the target LMO4. Functionally, we found that inhibiting circBACH2 expression decreased cell proliferation, migration, and invasion. Finally, down-regulating circBAC...Continue Reading

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Citations

Feb 9, 2021·Frontiers in Endocrinology·Xiaoqin Xu, Jiexian Jing
Nov 6, 2020·BioMed Research International·Danhui WangYongjun Wu
Feb 16, 2020·Molecular Therapy. Nucleic Acids·Guojun WuXingsong Tian
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Methods Mentioned

BETA
immunoprecipitation
pull down
RIP
transfection
xenograft
PCR
Assay
reverse transcription PCR
xenografts

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