Circular RNA circCTNNA1 promotes colorectal cancer progression by sponging miR-149-5p and regulating FOXM1 expression.

Cell Death & Disease
Pengju ChenAiwen Wu

Abstract

Circular RNAs (circRNAs) are an emerging class of non-coding RNAs, identified to participate in multiple malignancies. Nevertheless, the clinical significance, biological function, and regulatory mechanisms of circRNAs in colon cancer (CC) remain largely unclear. In this study, the circRNA expression profile in CC and matched normal tissues was analyzed using circRNA microarrays. A novel circRNA, circCTNNA1, was significantly upregulated in CC, and its level was associated with advanced tumor-node-metastasis stage and poor prognosis of patients with CC. Functional experiments, including Cell Counting Kit-8, colony formation, 5-ethynyl-2'-deoxyuridine, transwell, wound healing, flow cytometric analysis, and in vivo tumorigenesis assay were then performed to investigate the oncogenic role of circCTNNA1. The results revealed that circCTNNA1 promoted CC cell proliferation, migration, and invasion in vitro and in vivo. Mechanistically, RNA pull-down, RNA immunoprecipitation, dual-luciferase reporter assays, and fluorescent in situ hybridization were performed to unveil that circCTNNA1 can serve as a competing endogenous RNA of miR-149-5p to counteract the suppressive effect of miR-149-5p on downstream target Forkhead Box M1 (FOXM1)....Continue Reading

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Citations

Feb 8, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jasni Viralippurath AshrafEyad Elkord
Jun 1, 2021·Frontiers in Cell and Developmental Biology·Yuting ZhuYao Xue
May 21, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardReza Vafaee
Jul 31, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ke-Da YangZhi-Jun Zeng
Jul 1, 2021·Oncology Letters·Mingying Zhang, Shubin Wang

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Methods Mentioned

BETA
flow cytometry
xenograft
pull-down
immunoprecipitation
pull down
transfection
electrophoresis

Software Mentioned

R
SPSS
TargetScan
clusterProfiler
GraphPad Prism
miRanda
CircInteractome

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