Abstract
Oral squamous cell carcinoma (OSCC) is a pathological type of oral cancer, which accounts for over 90% of oral cancers. It has been widely shown that circRNA is involved in the regulation of multiple malignant oral diseases including OSCC. However, the mechanism underlying how circRNA regulates OSCC is still not clearly elucidated. In this article, we report circFOXO3 promotes tumor growth and invasion of OSCC by targeting miR-214 which specifically degrades the lysine demethylase 2A (KDM2A). CircRNA sequencing was conducted in OSCC tumor and tumor-side tissues, and the expression of circFOXO3 is found to be markedly increased in tumor tissues. CircFOXO3 is also highly expressed in several OSCC cell lines compared with human oral keratinocytes. Transwell assay and colony formation showed that knockdown of circFOXO3 prevents the invasion and proliferation of oral cancer cells. Via bioinformatic research, miR-214 was found to be the target of circFOXO3 and correlate well with circFOXO3 both in vitro and in vivo. KDM2A was then validated by database analysis and luciferase assay to be the direct target of miR-214. KDM2A helps to promote tumor invasiveness and proliferation of OSCC. Collectively, our results proved that circFOXO3 s...Continue Reading
References
Mar 29, 2005·Oral Oncology·S E ScottM McGurk
Dec 20, 2005·Nature·Yu-ichi TsukadaYi Zhang
Oct 7, 2008·Nature Structural & Molecular Biology·Jin HeYi Zhang
Apr 27, 2010·Molecular Cell·Neil P BlackledgeRobert J Klose
Mar 7, 2013·The EMBO Journal·Matthias W Hentze, Thomas Preiss
Nov 10, 2013·The Journal of Clinical Investigation·Klaus W WagnerMin Gyu Lee
Jan 17, 2014·Nature·Yvonne TayPier Paolo Pandolfi
Apr 1, 2014·Cell·Thomas R Cech, Joan A Steitz
Aug 17, 2014·Genes & Development·Chi-Lin TsaiJohn A Tainer
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Sep 24, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yufeng HuangWangkun Lu
Dec 15, 2015·Oncogene·W YangB B Yang
Mar 10, 2017·Cell Cycle·Wei-Yang Lu
Mar 30, 2017·International Journal of Oncology·Kengo OkamotoMakoto Tsuneoka
Sep 25, 2017·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Alessandro Menna AlvesMarcelo Lazzaron Lamers
Jul 31, 2018·Nucleus·Tomáš VacíkIvan Raška
Jan 24, 2019·Clinical Oral Investigations·Ali-Farid SafiMatthias Kreppel
Jun 13, 2019·Journal of Cellular Physiology·Xing ChenJihao Ren
Sep 11, 2019·Neuro-oncology·Shuai ZhangYongming Qiu
Oct 2, 2019·Cell Death and Differentiation·Wei ZhaoJie Wu
Apr 29, 2020·Cancers·Ion CristóbalJesús García-Foncillas
May 24, 2020·Gene·Tian XiangGao Liu
Nov 19, 2020·Journal of Cellular and Molecular Medicine·Jian-Lin ZhouQiong-Jie Hu