Circular RNA circHIPK3 Promotes the Proliferation and Differentiation of Chicken Myoblast Cells by Sponging miR-30a-3p

Cells
Biao ChenQinghua Nie

Abstract

Circular RNAs and microRNAs widely exist in various species and play crucial roles in multiple biological processes. It is essential to study their roles in myogenesis. In our previous sequencing data, both miR-30a-3p and circular HIPK3 (circHIPK3) RNA, which are produced by the third exon of the HIPK3 gene, were differentially expressed among chicken skeletal muscles at 11 embryo age (E11), 16 embryo age (E16), and 1-day post-hatch (P1). Here, we investigated their potential roles in myogenesis. Proliferation experiment showed that miR-30a-3p could inhibit the proliferation of myoblast. Through dual-luciferase assay and Myosin heavy chain (MYHC) immunofluorescence, we found that miR-30a-3p could inhibit the differentiation of myoblast by binding to Myocyte Enhancer Factor 2 C (MEF2C), which could promote the differentiation of myoblast. Then, we found that circHIPK3 could act as a sponge of miR-30a-3p and exerted a counteractive effect of miR-30a-3p by promoting the proliferation and differentiation of myoblasts. Taking together, our data suggested that circHIPK3 could promote the chicken embryonic skeletal muscle development by sponging miR-30a-3p.

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Citations

Apr 24, 2019·International Journal of Molecular Sciences·Christine VoellenkleFabio Martelli
Aug 1, 2019·Acta Physiologica·Rui ChenYanling She
Sep 11, 2019·International Journal of Molecular Sciences·Karol CzubakMarzena Wojciechowska
Aug 16, 2019·Cells·Pengpeng ZhangYongjie Xu
Aug 18, 2020·Stem Cells International·Fanglin WangJun Fan
Jun 30, 2019·Cells·Xiaoyue LiShengwei Hu
Jul 8, 2020·Cells·Jesús Beltrán-GarcíaJosé Luis García-Giménez
Nov 17, 2020·Frontiers in Genetics·Dora Zucko, Kathleen Boris-Lawrie
Sep 12, 2020·Reproduction in Domestic Animals = Zuchthygiene·Yong HuangXihong Sun
Feb 16, 2021·Frontiers in Cell and Developmental Biology·Hongmei LuoBo Zuo
Jul 9, 2020·Molecular Therapy. Methods & Clinical Development·Shuyue RenGuanhua Du
Mar 23, 2021·Journal of Cellular Physiology·Mengjin GaoXihui Sheng
May 13, 2021·Cancer Cell International·Yihan Fu, Hong Sun
Jul 21, 2021·International Journal of Molecular Sciences·Golam RbbaniJorge M O Fernandes
Nov 6, 2021·Experimental and Therapeutic Medicine·Guoding CaoXusheng Li

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Datasets Mentioned

BETA
GSE89355

Methods Mentioned

BETA
PCR
electrophoresis
transfection
Assay
fluorescence microscopy
flow cytometry

Software Mentioned

DNASTAR
Photoshop Photoshop
Image J
FlowJo7
RNAhybrid
Premier Primer

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