Circular RNA circLMF1 regulates PDGF-BB-induced proliferation and migration of human aortic smooth muscle cells by regulating the miR-125a-3p/VEGFA or FGF1 axis.

Clinical Hemorheology and Microcirculation
Yanping YangYunfeng Pang

Abstract

Atherosclerosis is a major cause of cardiovascular disease, in which vascular smooth muscle cells (VSMCs) proliferation and migration play a vital role. Circular RNAs (circRNAs) have been reported to be correlated with the VSMCs function. Therefore, this study is designed to explore the role and mechanism of circRNA lipase maturation factor 1 (circLMF1) in Human aortic VSMCs (HASMCs). The microarray was used for detecting the expression of circLMF1 in proliferative and quiescent HASMCs. Levels of circLMF1, microRNA-125a-3p (miR-125a-3p), vascular endothelial growth factor A (VEGFA), and fibroblast growth factor 1 (FGF1) were determined by real-time quantitative polymerase chain reaction (RT-qPCR). Cell viability, cell cycle progression, and migration were assessed by Cell Counting Kit-8 (CCK-8), flow cytometry, wound healing, and transwell assays, respectively. Western blot assay determined proliferating cell nuclear antigen (PCNA), Cyclin D1, matrix metalloproteinase (MMP2), osteopontin (OPN), VEGFA, and FGF1 protein levels. The possible interactions between miR-125a-3p and circLMF1, and miR-125a-3p and VEGFA or FGF1 were predicted by circbank or targetscan, and then verified by a dual-luciferase reporter, RNA Immunoprecipitat...Continue Reading

References

Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
Jan 18, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Chunxiang ZhangTarun B Patel
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Aug 22, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marco CattaruzzaMarkus Hecker
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Mar 10, 2015·RNA Biology·Ling-Ling Chen, Li Yang
Jun 9, 2015·Cancer Letters·Shibin QuHaimin Li
Feb 20, 2016·Circulation Research·Martin R BennettGary K Owens
Feb 26, 2016·Nature Reviews. Molecular Cell Biology·Ling-Ling Chen
Mar 6, 2016·Journal of Medical Genetics·Yonghua ChenXubao Liu
Sep 28, 2016·Journal of Biotechnology·Franceli Rodrigues KulcheskiRogerio Margis
Dec 16, 2016·Thrombosis and Haemostasis·Donato Santovito, Christian Weber
Jan 28, 2017·European Journal of Preventive Cardiology·Robert ClarkeUNKNOWN China Kadoorie Biobank Collaborative Group
Dec 22, 2017·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Lei ZhangRuili He
Jul 31, 2018·Molecular Cell·Xiang LiLing-Ling Chen
Sep 19, 2018·Cellular Signalling·Emma L LowAngela C Bradshaw
Oct 21, 2018·Journal of Cellular Physiology·M-Ashraf AlteshaXiufen Zheng
Mar 4, 2019·Trends in Pharmacological Sciences·Niu NiuZheng-Gen Jin
Aug 8, 2019·Cardiovascular Research·Francesca FasoloJason L Johnson
Aug 10, 2019·Nature Reviews. Genetics·Lasse S KristensenJørgen Kjems
Sep 10, 2019·Journal of Molecular and Cellular Cardiology·Wei HuShenming Wang
May 26, 2020·Life Sciences·Qidong CaoChunli Song

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