Circular RNA CircMAP3K5 Acts as a MicroRNA-22-3p Sponge to Promote Resolution of Intimal Hyperplasia via TET2-Mediated SMC Differentiation

Circulation
Zhi ZengWai Ho Tang

Abstract

Background: Aberrant expression of circular RNA (CircRNA) contributes to human diseases. CircRNAs regulate gene expression by sequestering specific microRNAs (miRNAs). In this study, we investigated whether CircMAP3K5 could act as a competing endogenous miR-22-3p sponge and regulate neointimal hyperplasia. Methods: CircRNA profiling from genome-wide RNA sequencing data was compared between human coronary artery smooth muscle cells (HCASMCs) treated with or without PDGF. Expression levels of circular MAP3K5 (CircMAP3K5) was assessed in human coronary arteries from autopsies on patients with dilated cardiomyopathy (DCM) or coronary heart disease (CHD). The role of CircMAP3K5 in intimal hyperplasia was further investigated in mice with AAV9-mediated CircMAP3K5 transfection. SMC-specific Tet2 knockout mice and global miR-22-3p knockout mice were used to delineate the mechanism by which CircMAP3K5 attenuated neointimal hyperplasia using the femoral arterial wire injury model. Results: RNA sequencing demonstrated that treatment with PDGF-BB significantly reduced expression of CircMAP3K5 in HCASMCs. Wire-injured mouse femoral arteries and diseased arteries from CHD patients (where PDGF-BB is increased) confirmed in vivo downregulation ...Continue Reading

References

Sep 1, 1995·Circulation Research·S M SchwartzE R O'Brien
Mar 4, 2009·Nature Cell Biology·Julia WinterSven Diederichs
Jan 22, 2011·Journal of the American College of Cardiology·John F O'SullivanNoel M Caplice
May 3, 2011·The Journal of Surgical Research·Divya PankajakshanDevendra K Agrawal
Oct 2, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Seppo Ylä-Herttuala
Mar 1, 2013·Nature·Sebastian MemczakNikolaus Rajewsky
Dec 7, 2013·Expert Review of Clinical Pharmacology·Florence SalmonHarald Petry
May 9, 2014·Nature Biotechnology·William R Jeck, Norman E Sharpless
Sep 23, 2014·Molecular Cell·Reut Ashwal-FlussSebastian Kadener
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Jul 15, 2015·Biochimica Et Biophysica Acta·Karoline K EbbesenThomas B Hansen
Feb 20, 2016·Circulation Research·Martin R BennettGary K Owens
May 6, 2016·Frontiers in Molecular Neuroscience·Yiye Shao, Yinghui Chen
Aug 20, 2016·Nature Communications·Lesca M HoldtDaniel Teupser
Nov 24, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Jianhui ZhuangWenhui Peng
Jan 16, 2019·The Journal of Clinical Investigation·Zhi ZengWai Ho Tang
Feb 3, 2019·Nature Reviews. Drug Discovery·Dan WangGuangping Gao
May 14, 2019·Frontiers in Pharmacology·George Haddad, Johan M Lorenzen
Nov 14, 2019·Molecular Therapy. Methods & Clinical Development·Anca RemesAndreas H Wagner

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Citations

Apr 4, 2021·Cancers·Sergiu PascaCiprian Tomuleasa
May 1, 2021·Frontiers in Cardiovascular Medicine·Ling TangWuqiang Zhu
Jun 3, 2021·Biomedicines·Yuanyuan LiangLiling Tang
Oct 21, 2021·Chinese Medical Journal·Yi-Ping TangYu-Feng Qing

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