Circular RNA circVEGFC accelerates high glucose-induced vascular endothelial cells apoptosis through miR-338-3p/HIF-1α/VEGFA axis.

Aging
Hua WeiHongmian Li

Abstract

More and more findings illustrate the critical roles of circular RNA (circRNA) in diabetes mellitus (DM) and its complications. A major pathological characteristic for DM is the apoptosis of endothelial cells (ECs) induced by high glucose (HG), however, the function of circRNA in the ECs' phenotypes is still elusive. Here, this study identified an up-regulated circRNA (circVEGFC) in the HG-induced human umbilical vein endothelial cells (HUVECs). Functionally, knockdown of circVEGFC alleviated the apoptosis and recovered the proliferation in HUVECs induced by HG administration. Mechanistically, circVEGFC functioned as the sponge of miR-338-3p, and miR-338-3p was found to target the 3'-Untranslated Regions (3'-UTR) of hypoxia inducible factor 1 alpha (HIF-1α). HIF-1α, a critical transcription factor in DM, could activate the transcription of vascular endothelial growth factor A (VEGFA) and promote its protein product. In conclusion, these findings reveal the promotion of circVEGFC/miR-338-3p/HIF-1α/VEGFA axis in the HG-induced ECs' apoptosis, providing a potential treatment strategy for ECs' damage in DM.

References

May 26, 2017·Molecular Cancer·Shujuan MengMinghua Wu
Sep 28, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chen-Ye LiBo Yu
Dec 31, 2017·Investigative Ophthalmology & Visual Science·Shu-Jie ZhangBiao Yan
Jul 31, 2018·Molecular Cell·Xiang LiLing-Ling Chen
Nov 21, 2018·Biochemical and Biophysical Research Communications·Ying CaoRong Lu
Mar 13, 2019·Molecular Therapy. Nucleic Acids·Duc-Hiep BachAnil K Sood
Mar 27, 2019·Vascular Pharmacology·Nicolas JaéStefanie Dimmeler
May 15, 2019·Molecular Therapy. Nucleic Acids·Jie WuWei Zhao
Aug 14, 2019·International Journal of Nursing Sciences·Roger CarpenterKendra Barker
Oct 28, 2019·The American Journal of the Medical Sciences·Nicoleta D SoraAlicia J Jenkins
Nov 7, 2019·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Sami Hamdan AlzahraniWael Hassan Bakhamees

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Citations

Jan 10, 2021·Life Sciences·Sepideh MirzaeiMichael R Hamblin
Jul 13, 2021·Frontiers in Cardiovascular Medicine·Tiqun YangZhan-Peng Huang

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Methods Mentioned

BETA
transfection
Flow cytometry
Fluorescence
immunoprecipitation
PCR
Assay

Software Mentioned

GraphPad Prism
SPSS

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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This feed focuses on aging epidemiology and genetic, epigenetic, and proteomic aspects underlying aging, as well as aging- associated biomarkers. Here the latest research in this domain.

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