Circular RNA circZNF566 promotes hepatocellular carcinoma progression by sponging miR-4738-3p and regulating TDO2 expression.

Cell Death & Disease
Shanbao LiJunming Xu

Abstract

As a recently discovered noncoding RNA, circular RNAs (circRNAs) have been identified to play key roles in cancer biology; however, the detailed functions and mechanisms of circRNAs in hepatocellular carcinoma (HCC) remain largely unclarified. RNA-seq analysis was used to screen the expression profiles of circRNAs in HCC. CircZNF566 expression in HCC tissues and cell lines was detected by qRT-PCR. In vitro CCK-8, colony formation, wound healing, transwell migration, and invasion assays and in vivo tumorigenesis and metastasis assays were conducted to determine the functions of circZNF566. Luciferase reporter, RNA immunoprecipitation (RIP) and RNA pull-down assays were also performed to confirm the relationship between circZNF566 and miR-4738-3p. Bioinformatics analysis and luciferase reporter assays were employed to determine whether miR-4738-3p regulates tryptophan 2,3-dioxygenase (TDO2) expression. Finally, immunohistochemistry (IHC) was used to detect the level of TDO2 and determine its prognostic value. CircZNF566 was significantly upregulated in HCC tissues and cell lines. High circZNF566 expression in HCC tissues was positively correlated with clinicopathological features and poor prognosis. Functionally, in vitro experim...Continue Reading

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Citations

Feb 6, 2021·Journal of Hepatocellular Carcinoma·Abdullah ElyPatrick Arbuthnot
Oct 4, 2020·Biochemical and Biophysical Research Communications·Ranran SunGang Dong
Sep 1, 2021·Journal of Hematology & Oncology·Hao ShenXiujun Cai
Oct 17, 2021·Journal of Hematology & Oncology·Yiyin ZhangHui Lin

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Methods Mentioned

BETA
RNA-seq
pull-down
RIP
xenograft
surgical resection
Tandem Repeat
PCR
protein assay
electrophoresis
immunoprecipitation

Software Mentioned

miWalk
TargetScan
SPSS

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