Circular RNA CpG island hypermethylation-associated silencing in human cancer.

Oncotarget
Humberto J FerreiraM Esteller

Abstract

Noncoding RNAs (ncRNAs), such as microRNAs and long noncoding RNAs (lncRNAs), participate in cellular transformation. Work done in the last decade has also demonstrated that ncRNAs with growth-inhibitory functions can undergo promoter CpG island hypermethylation-associated silencing in tumorigenesis. Herein, we wondered whether circular RNAs (circRNAs), a type of RNA transcripts lacking 5'-3' ends and forming closed loops that are gaining relevance in cancer biology, are also a target of epigenetic inactivation in tumors. To tackle this issue, we have used cancer cells genetically deficient for the DNA methyltransferase enzymes in conjuction with circRNA expression microarrays. We have found that the loss of DNA methylation provokes a release of circRNA silencing. In particular, we have identified that promoter CpG island hypermethylation of the genes TUSC3 (tumor suppressor candidate 3), POMT1 (protein O-mannosyltransferase 1), ATRNL1 (attractin-like 1) and SAMD4A (sterile alpha motif domain containing 4A) is linked to the transcriptional downregulation of both linear mRNA and the hosted circRNA. Although some circRNAs regulate the linear transcript, we did not observe changes in TUSC3 mRNA levels upon TUSC3 circ104557 overexp...Continue Reading

References

Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H L SangerA K Kleinschmidt
Apr 5, 2002·Nature·Ina RheeBert Vogelstein
Jul 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Jun 10, 2005·Nature·Jun LuTodd R Golub
May 19, 2006·Cancer Research·Jingmin ShuJean-Pierre J Issa
Feb 20, 2007·Cancer Research·Amaia LujambioManel Esteller
May 19, 2007·Science·Philipp KapranovThomas R Gingeras
Mar 14, 2008·The New England Journal of Medicine·Manel Esteller
May 20, 2011·Epigenetics : Official Journal of the DNA Methylation Society·Juan SandovalManel Esteller
May 24, 2012·RNA Biology·Humberto J FerreiraManel Esteller
Sep 17, 2013·Molecular Cell·Yang ZhangLing-Ling Chen
Sep 23, 2014·RNA·Petar GlažarNikolaus Rajewsky
Oct 5, 2014·Genes & Development·Dongming Liang, Jeremy E Wilusz
Jan 21, 2015·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Blanka RobesovaDana Dvorakova
Feb 11, 2015·Nature Structural & Molecular Biology·Zhaoyong LiGe Shan
Apr 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·Raquel Boque-SastreSonia Guil
Jun 17, 2015·Nature Reviews. Genetics·Stefanie Jonas, Elisa Izaurralde
Jul 8, 2015·Biochimica Et Biophysica Acta·Julia Liz, Manel Esteller
Nov 6, 2015·Nature Medicine·Maite Huarte
Feb 26, 2016·Nature Reviews. Molecular Cell Biology·Ling-Ling Chen
Jun 22, 2016·Cancer Research·Alexander A SvoronosFrank J Slack
Jul 12, 2016·Cell·Francesco IorioMathew J Garnett
Nov 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Angel Diaz-LagaresManel Esteller
Nov 26, 2016·Cell Death and Differentiation·William W DuBurton B Yang
Feb 9, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wenhao WengAjay Goel
Jun 8, 2017·Life Sciences·Lea Alonso-RangelDoris Cerecedo
Sep 21, 2017·Cellular and Molecular Life Sciences : CMLS·Kateřina VašíčkováPetr Vaňhara
Oct 11, 2017·Oncogene·L S KristensenJ Kjems
Oct 17, 2017·Experimental Cell Research·Lian-Ju LiDong-Qing Ye
Oct 27, 2017·Oncotarget·Dawei RongHongyong Cao
Nov 1, 2017·Genome Biology·Francesco P MarcheseMaite Huarte

❮ Previous
Next ❯

Citations

Aug 23, 2019·Genome Research·Muller FabbriGeorge A Calin
Aug 10, 2019·Nature Reviews. Genetics·Lasse S KristensenJørgen Kjems
Sep 22, 2018·Oncotarget·Mihnea P Dragomir, George A Calin
Nov 12, 2019·Frontiers in Oncology·Itzel Alejandra Hernández-RomeroErnesto Soto-Reyes
Feb 18, 2020·Molecular Cancer·Jikui SunJinhuan Wang
Mar 6, 2021·Molecular Therapy. Nucleic Acids·Xi YangYonghong Li
Feb 23, 2021·Frontiers in Cell and Developmental Biology·Sabah NisarMohammad Haris
Dec 17, 2021·Nature Reviews. Clinical Oncology·Lasse S KristensenJørgen Kjems

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE109676

Methods Mentioned

BETA
PCR
PCRs
glycosylation
electrophoresis
Feature Extraction
transfection

Software Mentioned

Volcano Plot
R
Bioconductor
Agilent Feature Extraction

Related Concepts

Related Feeds

Cancer Epigenetics & Methyl-CpG (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. Here is the latest research on cancer epigenetics and methyl-CpG binding proteins including ZBTB38.

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cancer Epigenetics

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics and Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.