Circular RNA expression in exosomes derived from breast cancer cells and patients

Epigenomics
Jin-Yan WangShanliang Zhong

Abstract

We aimed to explore the roles of circular RNAs (circRNAs) in breast cancer (BCa). RNA was extracted from exosomes and BCa cells and analyzed using the RNA sequencing technique or microarray. Compared with controls, 1147 and 1195 circRNAs were dysregulated in exosomes from metastatic and localized BCa patients, respectively. A total of 480 dysregulated circRNAs were found in metastatic patients compared with localized patients, and these dysregulated circRNAs were enriched in eight pathways. Compared with MCF-7 cells and their exosomes, there were 5842 and 1137 dysregulated circRNAs in MDA-MB-231 cells and exosomes, respectively, and 5 circRNAs were confirmed using real-time quantitative PCR. We identified a number of dysregulated circRNAs in exosomes from BCa cells and patients.

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Citations

Jan 28, 2020·Journal of Cellular Physiology·Shima JahaniRezvan Esmaeili
Jul 7, 2019·Molecular Cancer·Yangxia WangLiang Ming
May 28, 2020·Nature Reviews. Molecular Cell Biology·Killian O'BrienXandra O Breakefield
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Dec 17, 2020·OncoTargets and Therapy·Yuqi BaiTiejun Wang
Mar 18, 2020·Differentiation; Research in Biological Diversity·Yang WangGuoli Yang
Jul 11, 2020·Molecular Therapy. Nucleic Acids·Man WangKun Wang
Oct 3, 2020·European Journal of Medicinal Chemistry·Mingyuan LiPeng Yu
Mar 30, 2021·Frontiers in Cell and Developmental Biology·Xiao HeQi Zhang
May 8, 2021·Seminars in Cancer Biology·Ying ZhangHang Yin

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Methods Mentioned

BETA
RNA-seq
blood collection
Feature Extraction
PCR

Software Mentioned

GeneSpring
DESeq2
RNAhybrid
circPrimer
Agilent Feature Extraction
Cytoscape
miRanda
R
DAVID

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