Circular RNA HIPK3 regulates human lens epithelial cell dysfunction by targeting the miR-221-3p/PI3K/AKT pathway in age-related cataract.

Experimental Eye Research
Gangfeng CuiWenjuan Huang

Abstract

Circular RNA Homeodomain Interacting Protein Kinase 3 (circHIPK3) was found to involve in the pathogenesis of age-related cataract (ARC). Here, we further disclosed the related target genes and molecular mechanism of circHIPK3 in the ARC progression. The expression of circHIPK3, microRNA (miR)-221-3p was detected using the quantitative real-time polymerase chain reaction. Human lens epithelial cell (HLEC) proliferation and apoptosis were measured by 3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-di-phenytetrazoliumromide (MTT) assay and flow cytometry, respectively. Western blot was used to detect the levels of apoptosis-related proteins, and phosphoinositide 3-kinase (PI3K)/p-protein kinase B (AKT) pathway-related proteins. Levels of malondialdehyde (MDA) and glutathione peroxidase (GSH-PX) were measured by kits. The interaction between miR-221-3p and circHIPK3 was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation assay. CircHIPK3 was down-regulated while miR-221-3p was up-regulated in human lens epithelium samples of ARC patients. CircHIPK3 up-regulation or miR-221-3p down-regulation mediated the promotion of proliferation, inhibition of apoptosis, decrease of MDA level as well as increase of GSH-PX level in HL...Continue Reading

Citations

Feb 26, 2021·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Langen ZhuangGuoxi Jin
Apr 13, 2021·Frontiers in Immunology·Yves-Marie PersDanièle Noël
May 1, 2021·International Journal of Molecular Sciences·Chisato Kinoshita, Koji Aoyama
May 13, 2021·Cancer Cell International·Yihan Fu, Hong Sun
Jun 23, 2021·Investigative Ophthalmology & Visual Science·Wouter H G HubensTheo G M F Gorgels

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