Circular RNA Hsa_circ_0066755 as an Oncogene via sponging miR-651 and as a Promising Diagnostic Biomarker for Nasopharyngeal Carcinoma.

International Journal of Medical Sciences
Jian WangXiaojie Chen

Abstract

Background: Circular RNAs (circRNAs) represent a class of broad and diversified endogenous RNAs that regulate gene expressions in eukaryotes. Hsa_circ_006675 has been proven as an important circRNA molecule in nasopharyngeal carcinoma (NPC), however, its function still remains elusive. This study aims to discuss the biofunctions of hsa_circ_0066755 in NPC. Methods: We detected the expression levels of hsa_circ_0066755 in NPC patients by quantitative real-time polymerase chain reaction (qRT-PCR), and the corresponding ROC curves were plotted. Functional experiments including CCK-8, colony formation, Transwell assay and Xenograft experiment were conducted. Bioinformatics analysis was performed to seek miRNAs which might have binding sites with hsa_circ_0066755. Luciferase reporter assays were finally carried out to verify the binding sites. Results: We found significant increases of hsa_circ_0066755 in the plasma and tissues of the patients. Moreover, its levels were positively correlated with clinical staging (P=0.019). The receiver operating characteristic (ROC) analysis showed that the area under the curves (AUCs) of tissue and plasma hsa_circ_0066755 for distinguishing NPC from non-cancerous controls were 0.8537 and 0.9044, r...Continue Reading

Citations

Apr 6, 2021·Frontiers in Medicine·Mina WangPeng Wei
May 18, 2021·Frontiers in Oncology·Panchun LiZhaojian Gong
Jul 11, 2021·The Kaohsiung Journal of Medical Sciences·Ayiguli HareHai-Feng Wang
Aug 28, 2021·Molecular Medicine·Mei TaoShaoqing Ju

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Methods Mentioned

BETA
biopsy
transfection
electrophoresis

Software Mentioned

CircRNA
Quantity ONE

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