Circular RNA profile of breast cancer brain metastasis: identification of potential biomarkers and therapeutic targets

Epigenomics
Bo FuJianqiong Zhang

Abstract

To explore the circular RNA (circRNA) profile of breast cancer brain metastasis (BCBM). RNA-seq was performed to identify the circRNA expression profile of brain metastatic breast cancer cell line 231-BR, in comparison with its parental nonspecific metastatic cell line MDA-MB-231. A total of 215 upregulated and 191 downregulated circRNAs were identified. The expression levels of ten randomly selected majorly altered circRNAs were re-examined by real-time quantitative PCR and agarose gel electrophoresis, and the following alterations were verified: upregulation of hsa_circ_0001944, hsa_circ_0001481, hsa_circ_0000646, hsa_circ_0001006 and hsa_circ_0000732, and downregulation of hsa_circ_0001910, hsa_circ_0008285 and hsa_circ_0000002. CircRNA/miRNA analysis revealed that hsa_circ_0001944 may be involved in BCBM through sponging up miR-509 and interfering with its binding to the downstream targets. This study provides a leading and fundamental circRNA profile of BCBM.

References

Aug 14, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T YonedaR Nishimura
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Feb 6, 2010·Genome Biology·Matthew D YoungAlicia Oshlack
Mar 1, 2013·Nature·Sebastian MemczakNikolaus Rajewsky
Jan 20, 2016·Breast Cancer Research : BCR·Isabell WitzelHarriet Wikman
Dec 3, 2016·Nucleic Acids Research·Minoru KanehisaKanae Morishima
Jan 20, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mingchen ZhuFeng Yan
Mar 24, 2017·Briefings in Bioinformatics·Yuan GaoFangqing Zhao
Dec 31, 2017·Journal of Translational Medicine·Hans-Juergen SchultenMohammed H Al-Qahtani
Jan 14, 2018·Cancer Letters·Bing Chen, Shenglin Huang
Jan 27, 2018·Journal of Cancer Research and Clinical Oncology·Ming-Ming JiangQing-Guo Di
Feb 7, 2018·Pharmacology & Therapeutics·Bing HanHonghong Yao
Mar 29, 2018·Cancer Research·Mantang QiuLin Xu
Apr 3, 2018·Biochemical and Biophysical Research Communications·Lihua ZhongYingji Ma
Apr 9, 2018·Biochemical and Biophysical Research Communications·Weidong JiangFangying Yin
May 9, 2018·Biochemical and Biophysical Research Communications·Jianhua ZhangFenxia Li
May 29, 2018·Cancer Letters·Jie WuShouyu Wang

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Citations

Jul 31, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yan MaoHaibo Wang
Aug 26, 2020·Molecular Cancer·Xiaolong XuLei Wei
Jan 5, 2021·Frontiers in Bioengineering and Biotechnology·Yuxin RanHongbo Qi
Mar 6, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Mar 30, 2021·Frontiers in Cell and Developmental Biology·Xiao HeQi Zhang
May 31, 2021·Pathology, Research and Practice·Soudeh Ghafouri-FardSeyed Abdulmajid Ayatollahi
Aug 1, 2021·Journal of Clinical Laboratory Analysis·Mingyu ChuYucui Jin
Sep 7, 2021·Frontiers in Cell and Developmental Biology·Rui ZhouGuochang Liu
Jan 23, 2022·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Di ChenChengyu Luo

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Methods Mentioned

BETA
PCR
electrophoresis
RNA-seq
reverse transcription PCR
GTPase

Software Mentioned

Cytoscape
find
CIRI2
bowtie2
miRanda
CircRNA
DESeq R package
KOBAS
circ
SPSS18

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