Circular RNAs are differentially expressed in liver ischemia/reperfusion injury model

Journal of Cellular Biochemistry
Zhiqiang YeHong Deng

Abstract

Liver ischemia/reperfusion (I/R) injury has high mortality due to the intense inflammatory process occurs in the liver. However, the pathological mechanism underlying I/R injury is still not clear. Recent works showed that circular RNAs play critical roles in many human diseases. In this study, the occurrence of liver I/R injury was validated by an analysis of the blood samples and hematoxylin-eosin (HE) staining of liver tissues. Total RNA was purified and followed by RNA-seq in the purpose of screening the circRNAs in significant differentially expression, which were validated by quantitative PCR. GO and KEGG analysis were performed to determine the function of these differentially expressed circular RNAs. The circular structure of the circRNA was validated with gel electrophoresis and RNase R treatment. We found that some circular RNAs were differentially expressed in Liver I/R mouse models through bioinformatics analysis. These circular RNAs play roles in biological process, cellular component, and molecular function through GO analysis. Meanwhile, Hippo signaling pathway was found to be correlated with circular RNAs function in I/R models by KEGG analysis. To further validate bioinformatics data, two up-regulated and three...Continue Reading

References

Feb 5, 1999·Journal of Hepato-biliary-pancreatic Surgery·H Jaeschke
Aug 1, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A B LentschM J Edwards
Apr 12, 2001·Seminars in Liver Disease·M Selzner, P A Clavien
Jul 16, 2003·Journal of Gastroenterology and Hepatology·Narci C Teoh, Geoffrey C Farrell
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Mar 31, 2012·Transplantation Reviews·Hartmut Jaeschke, Benjamin L Woolbright
Mar 1, 2013·Nature·Sebastian MemczakNikolaus Rajewsky
Oct 23, 2013·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Patricia GenovésJosé Miguel Lloris-Carsi
Jun 8, 2014·Lasers in Medical Science·Mohammad Ashrafzadeh TakhtfooladiMohammadreza Khansari
Sep 18, 2014·Acta Cirúrgica Brasileira·Daniele Moraes LosadaOrlando Castro-e-Silva
Jan 29, 2016·Biochemical and Biophysical Research Communications·Shaowei LiShiro Takahara
Nov 26, 2016·Cell Death and Differentiation·William W DuBurton B Yang
May 10, 2017·Experimental Biology and Medicine·Kuei-Yang HsiaoShaw-Jenq Tsai
Jul 19, 2017·BioMed Research International·Xing-Ya GuoJian-Gao Fan
Sep 29, 2017·PloS One·Waqar K SaeedHyeon Tae Kang
Oct 4, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ai SadatomoMasafumi Takahashi
Oct 12, 2017·Oxidative Medicine and Cellular Longevity·Xing-Ya GuoJian-Gao Fan
Oct 27, 2017·Oncotarget·Dawei RongHongyong Cao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.