PMID: 9548413Apr 21, 1998Paper

Circulating CD34+ cells in cord blood and mobilized blood have a different profile of adhesion molecules than bone marrow CD34+ cells

European Journal of Haematology
K AsosinghM De Waele

Abstract

The expression of adhesion molecules was studied on CD34+ hematopoietic precursors in cord blood, bone marrow and mobilized blood. The samples were labeled in a double immunofluorescence procedure with a CD34 monoclonal antibody and with antibodies against maturation and differentiation antigens and adhesion molecules. Myeloid precursors formed the majority of the CD34+ cells in all samples. In bone marrow a separate cluster of B-cell precursors with low forward scatter was present. Nearly all CD34+ cells in normal bone marrow expressed VLA-4 and VLA-5, PECAM-1, LFA-3 and HCAM. The majority of the CD34+ cells also had LFA -1 and L-selectin on the surface membrane. A small subset was VLA-2, VLA-3, ICAM-1 or Mac-1 positive. CD34+ cells expressing the vitronectin receptor or the CD11c antigen were rare. Cord blood and mobilized blood CD34+ cells had a lower expression of VLA-2, VLA-3 and VLA-5 and a higher expression of LFA-1, ICAM-1 and L-selectin than bone marrow CD34+ cells. Except for LFA-1, this was not due to the presence of more myeloid precursors in these samples. Low beta1 integrin expression may lead to less adhesion to the extracellular matrix. High expression of L-selectin may facilitate interaction with endothelial ce...Continue Reading

References

Aug 1, 1992·The Journal of Clinical Investigation·J TeixidóP Anklesaria
Oct 1, 1992·Annals of Hematology·M A Reuss-BorstC A Müller
Apr 1, 1987·The Journal of Pediatrics·R D Christensen
Jul 1, 1994·British Journal of Haematology·B ThilaganathanG Morgan
May 1, 1993·The Journal of Experimental Medicine·W CraigP M Lansdorp
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T Papayannopoulou, B Nakamoto
Jul 1, 1994·British Journal of Haematology·H M JonesD C Linch
Jun 1, 1994·Critical Reviews in Oncology/hematology·C VerfaillieJ B McCarthy

❮ Previous
Next ❯

Citations

Sep 12, 2000·Journal of Hematotherapy & Stem Cell Research·B DaveJ E Talmadge
Nov 23, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·P PrankeJ Visser
Oct 10, 2008·Stem Cells and Development·Halvard BonigThalia Papayannopoulou
Feb 27, 2004·British Journal of Haematology·S M CreanR S Taichman
Jun 15, 1999·Pediatric Research·J M KoenigA B Deisseroth
Aug 22, 2003·British Journal of Haematology·Karen A WhitingRuth Pettengell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.