Circulating Cell-Free DNA as a Prognostic and Molecular Marker for Patients with Brain Tumors under Perillyl Alcohol-Based Therapy

International Journal of Molecular Sciences
Giselle FariaThereza Quirico-Santos

Abstract

Tumor infiltration into brain tissue usually remains undetected even by high-resolution imaging. Molecular markers are used to increase diagnostic accuracy, but with limited continuous monitoring application. We evaluated the potential of circulating cell-free DNA (cfDNA) as a molecular indicator of the response to therapy by the intranasal administration (ITN) of perillyl alcohol (POH) in brain tumors. The cohort included 130 healthy subjects arranged as control-paired groups and patients at terminal stages with glioblastoma (GBM, n = 122) or brain metastasis (BM, n = 55) from stage IV adenocarcinomas. Serum cfDNA was isolated and quantified by fluorimetry. Compared with the controls (40 ng/mL), patients with brain tumors before ITN-POH treatment had increased (p < 0.0001) cfDNA median levels: GBM (286 ng/mL) and BM (588 ng/mL). ITN-POH treatment was significantly correlated (rho = -0.225; p = 0.024) with survival of >6 months at a concentration of 599 ± 221 ng/mL and of.

References

Jun 1, 1987·European Journal of Cancer & Clinical Oncology·M StrounP A Maurice
May 20, 2000·Annals of the New York Academy of Sciences·M StrounP Anker
Jul 19, 2002·International Journal of Cancer. Journal International Du Cancer·Thierry LecomtePierre Laurent-Puig
Sep 26, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Gabriella SozziUgo Pastorino
Jan 20, 2004·Acta Neuropathologica·Stefan W SchneiderWerner Paulus
May 15, 2004·Clinical Chemistry·Nancy A ObuchowskiFrank H Wians
Nov 13, 2004·Clinical Chemistry·Luis J HerreraTony E Godfrey
Nov 17, 2006·Annals of the New York Academy of Sciences·Svetlana N TamkovichPavel P Laktionov
Dec 2, 2006·Biochimica Et Biophysica Acta·M Fleischhacker, B Schmidt
Oct 8, 2008·Annals of the New York Academy of Sciences·Peter B Gahan, R Swaminathan
Oct 8, 2008·Annals of the New York Academy of Sciences·Svetlana N TamkovichPavel P Laktionov
Mar 6, 2009·Cell Adhesion & Migration·Jong Bae ParkSeung-Hoon Lee
Apr 20, 2010·Journal of Cancer Research and Clinical Oncology·Clovis O da FonsecaThereza Quirico-Santos
May 13, 2011·Nature Reviews. Cancer·Heidi SchwarzenbachKlaus Pantel
Jul 16, 2011·Neuro-oncology·Christian la FougèreJörg-Christian Tonn
Sep 2, 2011·Journal of Molecular Neuroscience : MN·Wei ShiJian Chen
Oct 16, 2012·Biochemical Pharmacology·Luca PersanoGiampietro Viola
Nov 2, 2012·Clinical Chemistry·Robert R Langley, Isaiah J Fidler
Mar 5, 2013·Scientific Reports·Jan EggerRon Kikinis
Nov 10, 2013·Nature Medicine·Daniela F Quail, Johanna A Joyce
Oct 15, 2014·EMBO Molecular Medicine·Steven K CarlssonClaes Wahlestedt
Jul 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yuxuan WangChetan Bettegowda
Jul 30, 2015·The Cancer Journal·Isaiah J Fidler
Sep 25, 2015·Molecular Diagnosis & Therapy·Raghu AarthyThangarajan Rajkumar
Nov 20, 2016·The Journal of Molecular Diagnostics : JMD·Joanna Wang, Chetan Bettegowda
Mar 21, 2017·Archivum Immunologiae Et Therapiae Experimentalis·Aline C GomesThereza Quirico-Santos

❮ Previous
Next ❯

Citations

Oct 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Olga AntipovaAlexey Kopylov
Nov 2, 2019·British Journal of Cancer·Juliana Müller BarkChamindie Punyadeera
Dec 4, 2020·Expert Review of Molecular Diagnostics·O E BryzgunovaP P Laktionov
Mar 23, 2021·Frontiers in Immunology·Darel Martínez BedoyaDenis Migliorini
Jun 22, 2021·Frontiers in Oncology·Hamza AliMyron G Best

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging techniques
surgical resection
Assay

Software Mentioned

GraphPad Prism
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.