Circulating cell-free fetal messenger RNA levels after fetoscopic interventions of complicated pregnancies

American Journal of Obstetrics and Gynecology
May Lee TjoaJan A Deprest

Abstract

The aim of this study was to examine fetal gene expression in maternal plasma after fetoscopic intervention for twin-twin transfusion syndrome or congenital diaphragmatic hernia. Twelve women with pregnancies that were complicated by twin-twin transfusion syndrome and 10 women carrying fetuses with congenital diaphragmatic hernia were sampled before and sequentially after treatment. Levels of glyceraldehyde-3-phosphate dehydrogenase, human placental lactogen, and gamma globin messenger RNA were measured by real-time reverse transcriptase polymerase chain reaction amplification. At all time points, glyceraldehyde-3-phosphate dehydrogenase messenger RNA levels were higher in the congenital diaphragmatic hernia cases than in the twin-twin transfusion syndrome cases (P < .05), but during the immediate postoperative observation period, there were no significant changes in glyceraldehyde-3-phosphate dehydrogenase, human placental lactogen, or gamma globin messenger RNA levels in individual patients or patients who were grouped by procedure. Fetoscopic intervention of complicated pregnancies does not affect circulating fetal messenger RNA levels, which is in contrast to earlier observations that circulating fetal DNA levels increase a...Continue Reading

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Citations

Oct 31, 2006·Photomedicine and Laser Surgery
Mar 1, 2011·Expert Opinion on Medical Diagnostics·Eva ViscaSinuhe Hahn
Feb 12, 2013·Seminars in Pediatric Surgery·Amar Nijagal, Tippi C MacKenzie
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Mar 16, 2019·Current Stem Cell Reports·Amir Alhajjat, Aimen Shaaban

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