Circulating CXCR5⁺CD4⁺ T Follicular-Like Helper Cell and Memory B Cell Responses to Human Papillomavirus Vaccines

PloS One
Ken MatsuiLigia A Pinto

Abstract

Through the interaction of T follicular helper (Tfh) cells and B cells, efficacious vaccines can generate high-affinity, pathogen-neutralizing antibodies, and memory B cells. Using CXCR5, CXCR3, CCR6, CCR7, PD1, and ICOS as markers, Tfh-like cells can be identified in the circulation and be classified into three functionally distinct subsets that are PD1+ICOS+, PD1+ ICOS-, or PD1-ICOS-. We used these markers to identify different subsets of CXCR5+CD4+ Tfh-like cells in response to highly immunogenic and efficacious vaccines for human papillomaviruses (HPV): Cervarix and Gardasil. In this small study, we used PBMC samples from 11 Gardasil recipients, and 8 Cervarix recipients from the Vaccine Research Center 902 Study to examine the induction of circulating Tfh-like cells and IgD-CD38HiCD27+ memory B cells by flow cytometry. PD1+ICOS+ CXCR3+CCR6-CXCR5+CD4+ (Tfh1-like) cells were induced and peaked on Day (D) 7 post-first vaccination, but not as much on D7 post-third vaccination. We also observed a trend toward increase in PD1+ICOS+ CXCR3-CCR6-CXCR5+CD4+ (Tfh2-like) cells for both vaccines, and PD1+ICOS+ CXCR3-CCR6+CXCR5+CD4+ (Tfh17-like) subset was induced by Cervarix post-first vaccination. There were also minimal changes in th...Continue Reading

References

Aug 19, 1999·The Journal of Pathology·J M WalboomersN Muñoz
Dec 6, 2000·The Journal of Experimental Medicine·P SchaerliB Moser
Jun 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tatyana ChtanovaCharles R Mackay
Aug 10, 2004·Nature Immunology·Christopher D C AllenJason G Cyster
Mar 18, 2005·Annual Review of Immunology·Louise J McHeyzer-Williams, Michael G McHeyzer-Williams
Jul 11, 2006·Immunological Reviews·Ian J AmannaShane Crotty
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lukas BossallerKlaus Warnatz
Sep 22, 2006·Clinical and Vaccine Immunology : CVI·Julie E MartinBarney S Graham
Oct 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicole M HaynesJason G Cyster
Jan 5, 2008·Annual Review of Immunology·Cecile KingCharles R Mackay
Jan 30, 2008·Current Protocols in Cell Biology·Christopher B Buck, Cynthia D Thompson
Jan 29, 2009·The Journal of the Acoustical Society of America·Fangyi Chen, Alfred L Nuttall
Apr 18, 2009·Nature Protocols·James J MoonMarc K Jenkins
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Klaus WarnatzHermann Eibel
Apr 29, 2010·Science Translational Medicine·Shohei KoyamaKen J Ishii
Jan 12, 2011·Annual Review of Immunology·Jennifer L CannonsPamela L Schwartzberg
Feb 15, 2011·Annual Review of Immunology·Shane Crotty
Apr 8, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nina ChevalierCharles R Mackay
Jan 10, 2012·Annual Review of Immunology·Gabriel D Victora, Michel C Nussenzweig
Apr 17, 2012·Immunological Reviews·Mark J Shlomchik, Florian Weisel
Dec 5, 2012·Vaccine·John T SchillerSuzanne M Garland
Dec 5, 2012·Vaccine·Margaret StanleyConnie Trimble
Mar 15, 2013·Science Translational Medicine·Salah-Eddine BentebibelHideki Ueno
Jul 31, 2013·British Journal of Haematology·Ken MatsuiLigia A Pinto
Sep 14, 2013·Science·David Tarlinton, Kim Good-Jacobson
Oct 22, 2013·Immunity·Nathalie Schmitt, Hideki Ueno
Nov 6, 2013·Immunology and Cell Biology·Louis M Tsai, Di Yu
Feb 6, 2014·PLoS Pathogens·Kristin L BoswellRichard A Koup
Jul 8, 2014·Trends in Immunology·Nathalie SchmittHideki Ueno
Jul 9, 2014·Expert Review of Vaccines·Margaret A StanleyAnna R Giuliano
Dec 9, 2014·Human Vaccines & Immunotherapeutics·Douglas M HerrinJulie E Ledgerwood

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Citations

Feb 6, 2014·PLoS Pathogens·Kristin L BoswellRichard A Koup
Aug 19, 2015·PLoS Neglected Tropical Diseases·Xiaojun ChenChuan Su
Jul 8, 2014·Trends in Immunology·Nathalie SchmittHideki Ueno
Apr 8, 2014·Trends in Immunology·Franco Pissani, Hendrik Streeck
Jan 9, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marios KoutsakosKatherine Kedzierska
Mar 3, 2018·Nature Reviews. Cancer·Richard B S Roden, Peter L Stern
Dec 15, 2020·Vaccine·Athina ZachariaJason D Marshall
Mar 30, 2021·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Ming YangQianjin Lu
May 26, 2021·Human Vaccines & Immunotherapeutics·J HoesH E de Melker
Jun 22, 2021·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Patricia Suàrez-FernándezRocío Laguna-Goya
Aug 4, 2021·Vaccine·Hiroyoshi Doi, Tatsuya Kanto
Nov 4, 2021·Journal of Leukocyte Biology·Marios KoutsakosJennifer A Juno
Oct 25, 2021·Experimental Gerontology·Nicolás Lalinde-RuizCarlos Alberto Parra-López

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Methods Mentioned

BETA
FACS
FCS
ELISA
flow cytometry

Clinical Trials Mentioned

NCT01132859

Software Mentioned

FACSDiVA
DeNovo
FCS Express

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