Circulating diiodotyrosine: studies of its serum concentration, source, and turnover using radioimmunoassay after immunoextraction

The Journal of Clinical Endocrinology and Metabolism
H MeinholdK W Wenzel

Abstract

This report describes the application of a sensitive, specific, and reproducible RIA for diiodotyrosine (DIT) in human serum and metabolic studies on the source and kinetics of circulating DIT. Interference by cross-reactivity of T4 and other analogs was completely eliminated by isolation of DIT from serum with an efficient preparative immunoprecipitation technique. Mean (+/- SD) serum DIT levels were 161 +/- 133 pmol/liter (7.0 ng/100 ml) in 41 normal subjects, 64 +/- 30 pmol/liter in 46 pregnant women, 241 +/- 83 pmol/liter in the cord serum of 48 newborn infants, 542 +/- 494 pmol/liter in 22 hyperthyroid patients, and 101 +/- 71 pmol/liter in 15 hypothyroid patients. Mean values in pregnant, newborn and hyperthyroid subjects were significantly different from the normal mean. Very low DIT serum levels were found in four athyreotic patients during oral T4 substitution therapy, indicating that little DIT is formed by peripheral T4 degradation. In five normal subjects who received a single oral dose of 3 mg T4, serum DIT remained unchanged in one case and decreased in four cases. Radioimmunological measurements of DIT elimination from serum after the iv injection of 1 mg DIT in two normal volunteers gave MCRs of 103 and 133 lite...Continue Reading

Citations

Sep 1, 2005·Thyroid : Official Journal of the American Thyroid Association·Sing-Yung WuInder J Chopra
Mar 19, 2014·Best Practice & Research. Clinical Endocrinology & Metabolism·Ainhoa IglesiasJosé C Moreno
May 1, 1986·Clinical Endocrinology·D B RamsdenM C Sheppard
Jan 23, 2017·Molecular and Cellular Endocrinology·Anne H van der SpekAnita Boelen
Sep 1, 1983·Biomedical Mass Spectrometry·M N MohammedD B Ramsden
Dec 1, 1988·Scandinavian Journal of Clinical and Laboratory Investigation·J FaberC Bregengaard

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