Circulating DNA:anti-DNA complexes in systemic lupus erythematosus. Detection and characterization by ultracentrifugation

The Journal of Clinical Investigation
C Bruneau, J Benveniste


Although it is generally accepted that DNA:anti-DNA immune complexes play a significant role in the pathogenesis of tissue injury in systemic lupus erythematosus, their presence in the circulation is still a matter of controversy. In this study, we detected DNA:anti-DNA compexes by identification of both the antigen and(or) the antibody, the necessary requisites for immune complex definition, in 14 of 24 plasmas (7 of 11 patients). These antibodies were specific for native DNA and could be adsorbed by anti-immunoglobulin (Ig)G antisera. The DNA recovered was, at least in part, of low molecular weight. The presence of DNA:anti-DNA compexes was not related to high molecular weight IgG, cryoprecipitins, positive polyethylene glycol precipitation, or low plasma C3 levels. It was related significantly to low plasma C4 levels and to the presence of diffuse proliferative nephritis. The lack of correlation with other methods of detection of immune complexes and with the presence of heavy IgG (above 13 S) is in favor of the existence of other antigen-antibody systems (or aggregated immunoglobulins) in systemic lupus erythematosus plasmas. From the results, it appears that methods directed towards the demonstration of specific immune com...Continue Reading


May 14, 1999·Arthritis and Rheumatism·Z AmouraS Koutouzov
Jan 1, 1981·Rheumatology International·S ChitrabamrungE M Tan
Jan 1, 1982·Rheumatology International·T HelveO Wegelius
Jan 1, 1981·Rheumatology International·R P TaylorE L Wright
Jan 1, 1982·Springer Seminars in Immunopathology·T J Neale, C B Wilson
Oct 22, 2009·Rheumatology International·Fernando Martinez-ValleMiquel Vilardell-Tarres
Dec 30, 1982·Journal of Immunological Methods·R SmeenkL Aarden
Jan 1, 1991·Molecular Aspects of Medicine·S BlountJ Lunec
May 1, 1993·Free Radical Biology & Medicine·K FrenkelE Taioli
Apr 1, 1982·The Journal of Experimental Medicine·W Emlen, M Mannik
Sep 1, 1989·Annals of the Rheumatic Diseases·J C NossentA J Swaak
Dec 1, 1980·The Journal of Clinical Investigation·L Raptis, H A Menard
Mar 1, 1984·The Journal of Clinical Investigation·C R Steinman
Mar 1, 1990·Clinical Rheumatology·R SmeenkT Swaak
Jan 1, 1989·Critical Reviews in Clinical Laboratory Sciences·T M Phillips
Jan 1, 1980·Immunological Communications·E R De GrootC J van Oss
Jan 1, 1985·Scandinavian Journal of Rheumatology. Supplement·R SmeenkT Swaak
Nov 20, 2015·Nature Reviews. Rheumatology·David S Pisetsky
Feb 1, 1988·Kidney International·G J Fournié
Feb 1, 1985·Clinical Immunology and Immunopathology·S R PerR Heimer
Jul 1, 1985·Clinics in Dermatology·K B Kulick, M Reichlin
Jan 27, 2011·European Journal of Immunology·Hiroyuki YoshidaYoshinobu Takakura
Aug 1, 1982·Arthritis and Rheumatism·C A Dorsch, J Meyerhoff
Feb 1, 1985·Annals of the Rheumatic Diseases·M A FrenchN R Rowell
Feb 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·H SarvasM Leirisalo-Repo
Jan 19, 2018·Expert Reviews in Molecular Medicine·Peter CelecPeter Boor


Jan 1, 1979·Journal of Immunological Methods·J Benveniste, C Bruneau
Jan 1, 1976·The Journal of Clinical Investigation·A N TheofilopoulosF J Dixon
Jan 1, 1975·Scandinavian Journal of Rheumatology. Supplement·R J HarbeckE J Bardana
Jan 1, 1976·Journal of Immunological Methods·L AardenE R De Groot
Jul 1, 1975·The Journal of Experimental Medicine·A T SobelH J Müller-Eberhard
Jul 1, 1971·The Journal of Experimental Medicine·D KofflerH G Kunkel

Related Concepts

Centrifugation, Density Gradient
Immune complex
Lupus Erythematosus, Systemic
Hydrogen-Ion Concentration
DNA, Double-Stranded

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

© 2020 Meta ULC. All rights reserved