Abstract
Angiogenesis is increased in B-cell chronic lymphocytic leukemia (B-CLL). We wanted to quantify and characterize the circulating endothelial cells (CECs) in patients with B-CLL and correlate with plasma angiogenesis-related factors. Using a four-color flow cytometry, we prospectively analyzed the CEC in the whole blood of 20 healthy controls and 20 patients with B-CLL. We quantified (CD45-/CD31+/CD146+) and characterized the CECs according to whether they were apoptotic (annexin stain) or activated (CD106+). We also measured plasma levels of vascular endothelial growth factor (VEGF), fibroblast growth factor-2 (FGF-2), and thrombospondin-1 (TSP-1). Most patients (90%) had Rai stages 0-2 at the time of diagnosis. As a group, B-CLL patients had higher number of CECs (median of 26.5 cells/ml) compared (P = 0.04) to healthy controls (18.5 cells/ml). However, only four (20%) patients had elevated CEC counts, defined as >/=2 SD of the control mean (>/=53 cells/ml). The proportions of apoptotic (P = 0.83) and activated (P = 0.12) CECs were similar in both groups. B-CLL patients had higher FGF-2 (P < 0.001), lower TSP-1 (P = 0.004), and similar VEGF (P = 0.27) plasma levels. The number of CECs was not associated with Rai stage, absolut...Continue Reading
References
Nov 27, 1997·The New England Journal of Medicine·A SoloveyR P Hebbel
Aug 15, 2000·Leukemia·A R KiniL C Peterson
May 23, 2001·Blood·P MancusoF Bertolini
Feb 22, 2002·British Journal of Cancer·S MolicaG Digiesi
Oct 18, 2002·Blood·Stefano MolicaFranco Dammacco
Jul 15, 2003·Mediators of Inflammation·Joanna Gora-TyborTadeusz Robak
Dec 23, 2003·Leukemia Research·O BaireyE Rabizadeh
Jun 9, 2004·European Journal of Haematology·Stefano MolicaGiovanna Digiesi
Dec 25, 2004·Blood·Hong ZhangOlcay A Batuman
Feb 9, 2005·Kidney International·Mehmet KocMark S Segal
Feb 16, 2005·Thrombosis and Haemostasis·Andrew D BlannFrançoise Dignat-George
Nov 1, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Christopher G WillettGregory Y Lauwers
Nov 30, 2005·European Journal of Haematology·Agnieszka WierzbowskaPiotr Smolewski
Mar 18, 2006·Blood·Patrizia MancusoFrancesco Bertolini
Apr 18, 2006·Seminars in Oncology·Tait D Shanafelt, Neil E Kay
Oct 13, 2006·Nature Reviews. Cancer·Francesco BertoliniRobert S Kerbel
Citations
Jan 7, 2009·Leukemia & Lymphoma·Joanna Gora-TyborTadeusz Robak
Jan 14, 2009·Scandinavian Journal of Clinical and Laboratory Investigation·S MariucciM Danova
Sep 5, 2008·Critical Reviews in Oncology/hematology·Balazs DomeJozsef Tovari
Dec 8, 2010·Hematological Oncology·Linda KideryováMarek Trněný
Feb 19, 2010·Cancer·Gian Matteo RigolinAntonio Cuneo
Jul 9, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Arwa M AliMasakazu Toi
Jul 17, 2010·Blood·Darren S SigalAlan Saven