Abstract
Circulating exosomes containing donor HLA and lung-associated self-antigens (SAg) are thought to play an important role in allograft rejection after human lung transplantation. We characterized exosomes isolated from serum of 10 lung transplant recipients (LTxR) diagnosed with bronchiolitis obliterans syndrome (BOS) and compared them with exosomes isolated from serum of 10 stable LTxR. Lung-associated SAg (K-α-1-tubulin [Kα1T] and collagen V [Col-V]), MHC class II molecules, costimulatory molecules CD40, CD80, and CD86, and transcription factors class II MHC trans-activator, NF-κB, hypoxia-inducible factor 1-α, IL-1R-associated kinase 1, MyD88, and 20S proteasome were detected in exosomes from BOS, but not stable LTxR. In contrast, adhesion molecules were present in both groups. C57BL/6 mice immunized with exosomes from BOS but not stable LTxR demonstrated Ab to SAg (Col-V, 33.5 ± 15.7 versus 10.4 ± 6.4, p = 0.021; Kα1T, 925 ± 403 versus 317 ± 285, p = 0.044) and HLA (mean fluorescence intensity: BOS, 8450; stable, 632; p < 0.05). Furthermore, splenic lymphocytes demonstrated increased frequency of lung SAg-specific IL-17 (Col-V, 128 ± 46 versus 31 ± 21, p = 0.013; Kα1T, 194 ± 47 versus 67 ± 43, p = 0.014) and IFN-γ (Col-V, 165...Continue Reading
References
Nov 1, 1995·The Annals of Thoracic Surgery·S SundaresanG A Patterson
Jul 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·M Azizul HaqueDavid S Wilkes
Aug 18, 2005·Immunologic Research·Elbert KuoT Mohanakumar
Aug 8, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A BharatT Mohanakumar
Oct 24, 2006·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Iskander Al-GithmiAnthony Yim
May 9, 2007·Nature Cell Biology·Hadi ValadiJan O Lötvall
Oct 30, 2007·The Journal of Clinical Investigation·William J BurlinghamDavid S Wilkes
Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Sep 10, 2008·Proteomics·Richard J SimpsonJustin W E Lim
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naohiko FukamiThalachallour Mohanakumar
Sep 28, 2010·The Annals of Thoracic Surgery·Ankit BharatThalachallour Mohanakumar
Oct 26, 2010·The Annals of Thoracic Surgery·Ankit BharatThalachallour Mohanakumar
Mar 19, 2011·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Deepti SainiThalachallour Mohanakumar
Apr 28, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·L FanD S Wilkes
Oct 4, 2011·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Jason D ChristieMarshall I Hertz
Dec 24, 2011·Cellular Immunology·Venkataswarup TiriveedhiT Mohanakumar
May 10, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R R HachemT Mohanakumar
Jan 22, 2013·The Journal of Allergy and Clinical Immunology·Bettina LevänenÅsa M Wheelock
Sep 16, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·V SubramanianT Mohanakumar
Jan 17, 2015·Frontiers in Medicine·Tara K SigdelMinnie M Sarwal
Jul 25, 2015·Tissue Antigens·T KaukeH Winter
Dec 18, 2015·Science Translational Medicine·Mélanie DieudéMarie-Josée Hébert
May 29, 2016·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Cynthia E FisherAjit P Limaye
Jun 10, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M GunasekaranT Mohanakumar
Jun 28, 2016·The Journal of Clinical Investigation·Quan LiuAdrian E Morelli
Dec 13, 2016·Transplantation·Zhongping XuThalachallour Mohanakumar
Dec 13, 2016·Science Immunology·Jose MarinoGilles Benichou
Oct 11, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P R AguilarR R Hachem
Citations
Apr 17, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Mónica MacíasÁlvaro González
Aug 9, 2019·Transplant International : Official Journal of the European Society for Organ Transplantation·Angara SureshbabuThalachallour Mohanakumar
May 20, 2020·International Journal of Immunogenetics·Xiaohai Zhang, Nancy L Reinsmoen
Nov 11, 2019·Transplant International : Official Journal of the European Society for Organ Transplantation·Robin SchmitzJean Kwun
Mar 19, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Carrie L ButlerElaine F Reed
Jul 30, 2020·Scientific Reports·Francis MigneaultMarie-Josée Hébert
Aug 31, 2020·World Journal of Transplantation·Nobuyuki Yoshiyasu, Masaaki Sato
Oct 1, 2020·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Kellie J GoodletSofya Tokman
Oct 13, 2020·Current Opinion in Organ Transplantation·Annette M JacksonMichelle J Hickey
Nov 11, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Camila MacedoAngus W Thomson
Dec 2, 2020·The Journal of Thoracic and Cardiovascular Surgery·Billanna HwangMichael S Mulligan
Jun 9, 2020·The Journal of Thoracic and Cardiovascular Surgery·Brian Bateson, Victor van Berkel
Jan 16, 2021·Transplant International : Official Journal of the European Society for Organ Transplantation·Monal SharmaThalachallour Mohanakumar
Oct 16, 2020·Expert Review of Respiratory Medicine·Ashwini ArjunaThalachallour Mohanakumar
Feb 13, 2020·Cardiovascular Research·Bruna Lima CorreaJean-Sébastien Silvestre
Mar 19, 2021·Science Translational Medicine·Furong ZengAdrian E Morelli
Jul 11, 2019·The Journal of Thoracic and Cardiovascular Surgery·Thalachallour MohanakumarRoss M Bremner
Jun 18, 2018·Human Immunology·Monal SharmaT Mohanakumar
Feb 26, 2020·Transplant Immunology·Benjamin Renaud-PicardRomain Kessler
Apr 24, 2021·Frontiers in Immunology·Qingyuan ZhengXiao-Kang Li
Feb 3, 2019·The Journal of Thoracic and Cardiovascular Surgery·Toyofumi F Chen-Yoshikawa
Sep 6, 2020·Transplantation·Yoshihiro ItabashiT Mohanakumar
Nov 20, 2020·Transplantation·Tineke Kardol-Hoefnagel, Henny G Otten
Jul 1, 2020·ACS Omega·Sandhya BansalThalachallour Mohanakumar
Feb 9, 2020·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Muthukumar GunasekaranThalachallour Mohanakumar
Dec 4, 2021·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Mohammad RahmanThalachallour Mohanakumar