Circulating long noncoding RNA ZNFX1 antisense RNA negatively correlates with disease risk, severity, inflammatory markers, and predicts poor prognosis in sepsis patients

Medicine
Yahuan Xu, Bibo Shao

Abstract

This study aimed to investigate the correlation of long noncoding RNA (lncRNA) ZNFX1 antisense RNA (ZFAS1) with disease risk, severity, inflammation markers, and prognosis in sepsis patients.A total of 202 sepsis patients were consecutively enrolled, and 200 healthy volunteers were also recruited as healthy controls (HCs). Plasma samples of all patients and HCs were collected. LncRNA ZFAS1 expression was determined by quantitative polymerase chain reaction assay and inflammatory cytokines levels were detected by enzyme-linked immunosorbent assay.The median value of lncRNA ZFAS1 expression in sepsis patients was (0.639 [0.325-1.071]), which was lower compared to HCs (1.957 [0.876-3.245], P < .001], and receiver operating characteristics (ROC) curve revealed that lncRNA ZFAS1 expression had a good diagnostic value for sepsis with area under curve (AUC) of 0.814 (95% confidence interval [CI]: 0.771-0.857). Spearman test disclosed that lncRNA ZFAS1 expression was negatively correlated with Acute Physiology and Chronic Health Evaluation (APACHE) II score (r = -0.505, P < .001), and it was negatively associated with levels of C-creative protein (r = -0.241, P = .001), tumor necrosis factor-α (r = -0.253, P < .001), and interleukin (I...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 20, 2008·Nature Reviews. Immunology·Daniel RittirschPeter A Ward
Jan 31, 2013·Intensive Care Medicine·R P DellingerUNKNOWN Surviving Sepsis Campaign Guidelines Committee including The Pediatric Subgroup
Nov 10, 2013·Critical Care Medicine·Elizabeth K StevensonAllan J Walkey
Aug 11, 2015·Journal of Immunology Research·Ildikó LászlóJános Fazakas
Sep 29, 2015·American Journal of Respiratory and Critical Care Medicine·Carolin FleischmannUNKNOWN International Forum of Acute Care Trialists
Feb 24, 2016·JAMA : the Journal of the American Medical Association·Mervyn SingerDerek C Angus
Mar 25, 2016·Nature·Eleonora LeucciJean-Christophe Marine
Jun 18, 2016·Cell·Maninjay K AtianandKatherine A Fitzgerald
Feb 10, 2018·Bioscience, Biotechnology, and Biochemistry·Weiran XuHong Xu
Aug 12, 2018·Scientific Reports·Raquel AlmansaJesus F Bermejo-Martin

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Citations

Oct 13, 2020·Current Opinion in Infectious Diseases·Mildred A Iro, Sudha Priya Soundara Pandi
Apr 18, 2021·BMC Genomics·Xubin ZhengLixin Cheng
Jun 1, 2021·Frontiers in Cellular and Infection Microbiology·Wei WangTie-Ning Zhang
Aug 14, 2021·Experimental Cell Research·Furong LuYiming Shao

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Methods Mentioned

BETA
Enzyme-linked immunosorbent assay
ELISA

Software Mentioned

GraphPad Prism
SPSS
GraphPad

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