Circulating Maternal Total Cell-Free DNA, Cell-Free Fetal DNA and Soluble Endoglin Levels in Preeclampsia: Predictors of Adverse Fetal Outcome? A Cohort Study

Molecular Diagnosis & Therapy
Radwa Marawan AbdelHalimIman Atef Mandour

Abstract

The diagnosis of preeclampsia (PE) is based on the measurement of maternal blood pressure and proteinuria; however, these parameters are not used in the prediction of adverse fetal outcomes that may occur due to fetal stress. The plasma concentrations of total cell-free DNA (cf-DNA), cell-free fetal DNA (cff-DNA) and soluble endoglin (sEng) are higher in women with established PE than in normotensive controls, and the increase is particularly marked in those with severe PE. We aimed to evaluate the levels of cf-DNA, cff-DNA and sEng in pregnant Egyptian women with PE in order to assess the severity of the disease and to detect their potential utility in the future prediction of time of delivery and adverse fetal outcome. The study included 107 pregnant females with established PE during their third trimester (51 with mild PE and 56 with severe PE), together with 93 normotensive pregnant women. Absolute quantitation of the hemoglobin subunit beta (HBB) and testis-specific protein, Y-linked 1 (TSPY1) genes for the measurement of cf-DNA and cff-DNA in maternal blood, respectively, was carried out using real-time polymerase chain reaction (PCR) together with the measurement of serum sEng using ELISA. An almost twofold increase in c...Continue Reading

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Citations

Jul 30, 2017·International Journal of Molecular Sciences·Pei-Yin TsaiMei-Tsz Su
Nov 9, 2017·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Simeon EcheJagidesa Moodley
Mar 25, 2017·American Journal of Hypertension·Rocío Muñoz-HernándezPablo Stiefel
Oct 27, 2021·Expert Review of Molecular Diagnostics·Ana C Palei

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