Circulating microRNA-196a as a candidate diagnostic biomarker for chronic hepatitis C

Molecular Medicine Reports
Bo LiuHeng-Shu Zhang

Abstract

Previous studies have demonstrated the inhibitory effect of microRNA (miR)-196a on hepatitis C virus (HCV) expression in human hepatocytes. However, the clinical implications of aberrant miR-196a expression and the application of circulating miR-196a in the diagnosis and management of chronic hepatitis C (CHC) require further investigation. The present study aimed to examine the possibility of using serum miR-196a as a biomarker for CHC. The Affymetrix miRNA array platform was used for miRNA expression profiling in adenovirus (Ad)-HCV core-infected (HepG2-HCV) and Ad-enhanced green fluorescence protein (EGFP)-infected HepG2 cells (HepG2-control). miR-196a downregulation and levels were analyzed using stem-loop reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis of the sera of 43 patients with CHC and 22 healthy controls. A total of six miRNAs were identified as significantly different (≥ 1.5 fold; P ≤ 0.05) between the two groups. Of note, significant miR-196a downregulation was observed in HepG2-HCV as compared with HepG2‑EGFP. Furthermore, as compared with that of the healthy control group, serum miR-196a was demonstrated to be significantly lower in patients with CHC. In addition, analysis of the ...Continue Reading

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Citations

Dec 26, 2017·Texas Heart Institute Journal·Yan WangSong Zhang
Jul 19, 2018·Expert Review of Molecular Diagnostics·Sreelakshmi KothaKeyur Patel
Apr 20, 2018·European Journal of Medical Research·Huange ZhuMiaoxian Li
Apr 4, 2021·International Journal of Molecular Sciences·Asahiro MorishitaTsutomu Masaki
Jun 25, 2021·Experimental and Molecular Pathology·Milad BastamiZiba Nariman-Saleh-Fam

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Methods Mentioned

BETA
PCR

Software Mentioned

SAS
ComparativeMarkerSelection
GenePattern
SPSS

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