Circulating microRNA-762 upregulates colorectal cancer might through Wnt-1/β-catenin signaling

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Peng-Sheng LaiChung-Yu Chen

Abstract

Background A number of microRNAs (miRNAs) have been demonstrated to be correlated with the diagnosis, progression and prognosis of colorectal cancer (CRC). However, the key miRNAs and the associated signaling pathways that regulate the growth and metastasis of CRC remain unclear. Methods miRNA array was analyzed in CRC CT26 cell-transplanted BALB/c mice. The effects of miR-762 mimics and inhibitors on the growth, invasion, cell cycle, and regulatory pathways of CRC CT26 cells were assessed. Cell cycle and sub-G1 assay were collected from the treated CT26 cells. Finally, blood samples were collected from patients with CRC. Further analysis to compare miR-762 levels in blood samples collected from CRC patients and normal control donors. Patients’ basic data were retrieved from electronic medical records and analyzed for age, gender, tumor staging, and survival. Results The miRNA levels in mice with CRC cell implantation indicated that plasma mmu-miR-762 was upregulated. Transfection of mmu-miR-762 mimic to CT26 cells increased cell viability, invasion, and EMT, whereas transfection of mmu-miR-762 inhibitor decreased the above abilities. Cells treated with high-concentration mmu-miR-762 inhibitor induced cell cycle arrest at G0/G1...Continue Reading

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