Circulating microRNAs as novel biomarkers of ALK-positive nonsmall cell lung cancer and predictors of response to crizotinib therapy

Oncotarget
Liang-Liang LiXiaoqing Liu

Abstract

Circulating microRNAs are potential diagnostic and predictive biomarkers, but have not been investigated for patients with anaplastic lymphoma kinase (ALK)-positive lung cancer. In this exploratory study, we sought to identify potential plasma biomarkers for ALK-positive non-small cell lung cancer (NSCLC). A microRNA microarray was used to select ALK-related microRNAs in ALK-positive NSCLC (n = 3), ALK-negative NSCLC (n = 3), and healthy subjects (n = 3). Plasma levels of 21 microRNAs were differentially expressed for ALK-positive and ALK-negative NSCLC, including 14 down-regulated and 7 up-regulated microRNAs. We also identified 5s rRNA as the most stable endogenous control gene using geNorm and NormFinder algorithms. Candidate microRNAs in plasma from ALK-positive (n = 41) and ALK-negative NSCLC patients (n = 32) were quantified using real-time reverse transcriptase quantitative polymerase chain reaction. The expression levels of miR-28-5p, miR-362-5p, and miR-660-5p were all down-regulated in ALK-positive NSCLC, compared with ALK-negative NSCLC. The areas under the receiver operating characteristic curves of miR-28-5p, miR-362-5p, miR-660-5p, and 3-microRNAs panel were 0.873, 0.673, 0.760, and 0.876, respectively. The positi...Continue Reading

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Citations

Apr 3, 2019·International Journal of Molecular Sciences·Kuan-Li WuAnd Jen-Yu Hung
Dec 11, 2019·Diagnostics·Antonia HarangușMărioara Șimon
May 5, 2018·Journal of Clinical Medicine·Tomomi FujiiChiho Ohbayashi
Apr 22, 2018·Scientific Reports·Ivan A ZaporozhchenkoPavel P Laktionov
Jul 31, 2018·Journal of Cellular Biochemistry·Yongfen BaoShigang Shan
Mar 3, 2020·Life Sciences·Md Mahmodul Hasan Sohel

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Methods Mentioned

BETA
chips
Feature Extraction

Software Mentioned

GeneSpring
GraphPad Prism
NormFinder
Agilent Feature Extraction
geNorm
SPSS

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