Circulating MiR-374a-5p is a potential modulator of the inflammatory process in obesity

Scientific Reports
Ayo P DoumateyCharles N Rotimi

Abstract

Obese individuals without expected metabolic co-morbidities are referred to as metabolically healthy obese (MHO). The molecular mechanisms underlying this phenotype remain elusive. MicroRNAs may be involved in the MHO phenotype. To test this hypothesis, we screened 179 serum miRNAs in 20 African-American women (10 MHOs and 10 metabolically abnormal obese individuals -MAO). We identified 8 differentially expressed miRNAs (DEMs) with validation in an independent sample of 64 MHO and 34 MAO. Of the eight DEMs in the screening phase (p ≤ 0.05), miR-374a-5p remained significant (p = 0.04) with directional consistency in the validation sample. Ingenuity Pathway analysis revealed that miR-374a-5p putatively targeted 37 mRNAs (e.g. chemokines and transcription factors) which are members of canonical pathways involved in inflammation (IL-17A signaling) and lipid metabolism. Analysis restricted to adipocytes, the main source of circulating miRNAs in obesity, identified 3 mRNAs (CCL2, STEAP2, EN1) as the main target of miR-374a-5p. Evaluation of the 3 mRNAs in an independent sample showed that CCL2 was significantly downregulated (p = 0.0005). In summary, MiR-374a-5p is upregulated in MHO compared to MAO individuals and appears to show as...Continue Reading

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Citations

Jun 19, 2019·International Journal of Molecular Sciences·Francesca CirilloMaria Elisabeth Street
Oct 17, 2018·Current Medicinal Chemistry·Jacopo AgrimiVincenzo Lionetti
Jan 17, 2019·BioMed Research International·Yuxiang HuangYongqiang Li
Jul 31, 2019·International Journal of Molecular Sciences·Mohammed El HafidiRoxana Carbó
May 2, 2019·Frontiers in Endocrinology·Andrea G Izquierdo, Ana B Crujeiras
Nov 25, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Omar Ramos-LopezJ Alfredo Martinez
Apr 24, 2021·World Journal of Diabetes·Magdalena Paczkowska-Abdulsalam, Adam Kretowski

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Methods Mentioned

BETA
PCR
RNA-Seq

Software Mentioned

package
GenEX
NormFinder
BowTie2
IPA
SPSS Statistics
R
geNorm

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