Circulating miRNAs in diabetic kidney disease: case-control study and in silico analyses

Acta Diabetologica
Taís S AssmannDaisy Crispim

Abstract

The aim of this study was to investigate a miRNA expression profile in type 1 diabetes mellitus (T1DM) patients with DKD (cases) or without this complication (controls). Expression of 48 miRNAs was screened in plasma of 58 T1DM patients (23 controls, 18 with moderate DKD, and 17 with severe DKD) using TaqMan Low Density Array cards (Thermo Fisher Scientific). Then, five of the dysregulated miRNAs were selected for validation in an independent sample of 10 T1DM controls and 19 patients with DKD (10 with moderate DKD and 9 with severe DKD), using RT-qPCR. Bioinformatic analyses were performed to explore the putative target genes and biological pathways regulated by the validated miRNAs. Among the 48 miRNAs investigated in the screening analysis, 9 miRNAs were differentially expressed between DKD cases and T1DM controls. Among them, the five most dysregulated miRNAs were chosen for validation in an independent sample. In the validation sample, miR-21-3p and miR-378-3p were confirmed to be upregulated in patients with severe DKD, while miR-16-5p and miR-29a-3p were downregulated in this group compared to T1DM controls and patients with moderate DKD. MiR-503-3p expression was not validated. Bioinformatic analyses indicate that the f...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 30, 2005·Nucleic Acids Research·Jinmai JiangThomas D Schmittgen
Nov 10, 2007·Nucleic Acids Research·Sam Griffiths-JonesAnton J Enright
Sep 9, 2008·Journal of the American Society of Nephrology : JASN·Scott J HarveyJeffrey H Miner
May 6, 2009·Annals of Internal Medicine·Andrew S LeveyUNKNOWN CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration)
Sep 18, 2010·Clinical Chemistry·Jessica A WeberKai Wang
Jan 26, 2011·Annual Review of Pathology·Yashpal S KanwarSheldon Chen
Jun 11, 2011·Contributions to Nephrology·Eberhard RitzIvan Rychlík
Jul 26, 2011·Journal of the American Society of Nephrology : JASN·Wei QinHui Y Lan
Sep 6, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Calida S NealJonathan M Gleadle
Nov 19, 2011·Nature Reviews. Genetics·Manel Esteller
Nov 19, 2011·Journal of the American Society of Nephrology : JASN·Bo WangPhillip Kantharidis
Jan 5, 2012·Physiological Genomics·Alison J KriegelMingyu Liang
Jan 10, 2012·Science China. Life Sciences·Yuliang Feng, Xiyong Yu
Mar 30, 2012·Omics : a Journal of Integrative Biology·Guangchuang YuQing-Yu He
May 15, 2012·Clinical Journal of the American Society of Nephrology : CJASN·Johan M Lorenzen, Thomas Thum
Jun 21, 2012·PloS One·Filippo MartinoThomas Thum
May 1, 2013·Nature Reviews. Endocrinology·Claudiane Guay, Romano Regazzi
May 15, 2013·Current Diabetes Reports·Johanna K DiStefanoM Lucrecia Alvarez
May 18, 2013·Nucleic Acids Research·Maria D ParaskevopoulouA G Hatzigeorgiou
Sep 10, 2013·Seminars in Nephrology·Marpadga A ReddyRama Natarajan
Oct 11, 2013·International Journal of Nephrology and Renovascular Disease·Arthur Ck ChungHui Y Lan
Nov 14, 2013·PloS One·Federica BaruttaGabriella Gruden
Jan 11, 2014·European Journal of Endocrinology·Taís Silveira AssmannDaisy Crispim
Jan 28, 2014·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Richard J MacisaacGeorge Jerums
Mar 1, 2014·Journal of the American Society of Nephrology : JASN·Chun-Liang LinFeng-Sheng Wang
Apr 20, 2014·The Journal of Clinical Investigation·Claudia BangThomas Thum
May 2, 2014·Advances in Chronic Kidney Disease·Frank C Brosius, Richard J Coward
Nov 25, 2014·Nucleic Acids Research·Ioannis S VlachosArtemis G Hatzigeorgiou
Dec 30, 2014·Diabetes Care·UNKNOWN American Diabetes Association
Feb 5, 2015·American Journal of Physiology. Renal Physiology·Jae-Eun KimTae-Hwan Kwon
Apr 17, 2015·Annals of the New York Academy of Sciences·Mitsuo Kato, Rama Natarajan
May 15, 2015·Current Diabetes Reports·Girish N NadkarniSteven G Coca
May 23, 2015·Nature Reviews. Cancer·Shuibin Lin, Richard I Gregory
Aug 1, 2015·Nature Methods·Harsh Dweep, Norbert Gretz
Aug 13, 2015·ELife·Vikram AgarwalDavid P Bartel
Sep 28, 2015·Diabetologia·Roberto BassiPaolo Fiorina
Oct 18, 2015·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Nov 22, 2015·Nucleic Acids Research·Chih-Hung ChouHsien-Da Huang
Feb 26, 2016·Current Diabetes Reports·Merlin C Thomas

❮ Previous
Next ❯

Citations

Mar 21, 2020·Cell Biochemistry and Function·Mingzhe ShaoGuangming Chen
Nov 22, 2019·Cellular and Molecular Life Sciences : CMLS·Ivo F MachadoAnabela P Rolo
Jun 21, 2019·Journal of Diabetes Research·Jianqin WangXiaochun Zhou
Jan 17, 2020·International Journal of Molecular Sciences·Silvia GaravelliPaola de Candia
Feb 3, 2021·Acta Diabetologica·Federica BaruttaGabriella Gruden
Nov 20, 2020·International Journal of Cardiology·Fernando Luís ScolariAndreia Biolo
Jan 13, 2021·International Journal of Molecular Sciences·Ivo F MachadoAnabela P Rolo
Jul 3, 2021·International Journal of Molecular Sciences·Swayam Prakash SrivastavaDaisuke Koya
Aug 29, 2021·Pharmaceuticals·Swayam Prakash SrivastavaJulie E Goodwin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.