Circulating mitochondrial DNA in serum of patients with granulomatosis with polyangiitis

Clinical and Experimental Immunology
M P SurmiakM Sanak

Abstract

Neutrophil is a key cell in pathophysiology of granulomatosis with polyangiitis. Recently, neutrophil extracellular traps were described in this disease. Mitochondrial DNA is also released during traps formation. We measured circulating cell-free mitochondrial and genomic DNA in serum of patients with granulomatosis with polyangiitis. Subjects with the disease (14 active and 11 in remission stage) and 10 healthy controls were enrolled. Quantitative real-time polymerase chain reaction (PCR) was used to measure 79 base pairs (bp) and 230 bp mtDNA fragments. Alu repeats were quantified to evaluate abundance of nuclear DNA in serum at the presence of plasmid control. Both fragments of mtDNA (79 bp and 230 bp) and genomic DNA were elevated significantly in granulomatosis with polyangiitis compared to controls. Only the shorter 79 bp mtDNA correlated with active stage of granulomatosis with polyangiitis and clinical symptoms. A mechanism of extracellular release of mitochondrial DNA accompanies the active stage of the disease. Circulating mtDNA is extremely high in untreated patients. This suggests that biomarker properties of mtDNA are useful for monitoring of treatment.

References

Apr 25, 2001·Arthritis and Rheumatism·J H StoneUNKNOWN International Network for the Study of the Systemic Vasculitides (INSSYS)
Sep 12, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Naoyuki UmetaniDave S B Hoon
Jul 17, 2007·Nature Reviews. Microbiology·Volker Brinkmann, Arturo Zychlinsky
Nov 3, 2007·Annals of Internal Medicine·Javier D FinkielmanUNKNOWN WGET Research Group
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
May 19, 2009·Nature Medicine·Kai KessenbrockDieter E Jenne
Jul 18, 2009·Cell Death and Differentiation·S YousefiH U Simon
Nov 26, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Florian H PilsczekPaul Kubes
Dec 16, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ravi S KeshariMadhu Dikshit
Mar 10, 2012·PloS One·Mohamed AbdgawadThomas Hellmark
Jun 12, 2012·The Journal of Clinical Investigation·Axelle CaudrillierMark R Looney
Aug 7, 2012·Clinical and Experimental Immunology·R Kettritz
Aug 17, 2014·Journal of Critical Care·Daniel J McIlroyZsolt J Balogh
Jan 21, 2015·The Journal of Trauma and Acute Care Surgery·Daniel J McIlroyZsolt J Balogh

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Citations

Jul 26, 2016·Frontiers in Immunology·Daniel Söderberg, Mårten Segelmark
Jul 10, 2016·Cancer Metastasis Reviews·A R ThierryM Stroun
Mar 30, 2019·Journal of Leukocyte Biology·Alexandra A VitaNicholas A Pullen
Jan 4, 2018·Proceedings of the National Academy of Sciences of the United States of America·Björn IngelssonAnders Rosén

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