Circulating red cell volume and red cell survival can be accurately determined in sheep using the [14C]cyanate label

Pediatric Research
D M MockR G Strauss

Abstract

The sheep commonly serves as an animal model for investigation of human fetal and newborn erythropoiesis and red blood cell kinetics. Measurement of red cell volume (RCV) and survival (RCS) in sheep would be useful for studying mechanisms of neonatal anemia. Unfortunately 51Cr, the standard method for RCV, is not suitable for RCS in sheep because 51Cr leaves the red cell too rapidly. We developed and validated the permanent label [14C]cyanate as a method for measuring both RCV and RCS in sheep. In 19 sheep, RCV was determined simultaneously using [14C]cyanate and 51Cr. RCV determined by [14C]cyanate agreed almost perfectly with RCV by 51Cr; correlation coefficient = 0.990. The line of regression had a slope of 0.94 and an intercept of 40; these parameters are not significantly different from a line of identity. In nine sheep, RCS was determined using [14C]cyanate. Survival after d 1 accurately fit a model containing two components: 1) an early exponential loss likely related to damage caused by labeling and handling and 2) a linear decrease that reflected normal survival of undamaged red cells. Mean potential life span (MPL) determined from the linear phase was 114 +/- 12 d (mean +/- 1 SD). These results agree with reported MPL...Continue Reading

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Citations

May 23, 2007·Journal of Pharmacokinetics and Pharmacodynamics·Kevin J FreisePeter Veng-Pedersen
Oct 22, 2008·Journal of Pharmacokinetics and Pharmacodynamics·Kevin J FreisePeter Veng-Pedersen
Apr 5, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Matthew RosebraughPeter Veng-Pedersen
Jun 24, 2004·Archives of Disease in Childhood. Fetal and Neonatal Edition·N AladangadyC A J Wardrop
Jul 4, 2008·Pediatric Research·Donald M MockJohn A Widness
Sep 3, 2013·Vox Sanguinis·G SimonovaY L Fung
Sep 27, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·R A BraceD M Mock

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