Circulating RNA transcripts identify therapeutic response in cystic fibrosis lung disease.

American Journal of Respiratory and Critical Care Medicine
M T SaavedraJerry A Nick

Abstract

Circulating leukocyte RNA transcripts are systemic markers of inflammation, which have not been studied in cystic fibrosis (CF) lung disease. Although the standard assessment of pulmonary treatment response is FEV(1), a measure of airflow limitation, the lack of systemic markers to reflect changes in lung inflammation critically limits the testing of proposed therapeutics. We sought to prospectively identify and validate peripheral blood leukocyte genes that could mark resolution of pulmonary infection and inflammation using a model by which RNA transcripts could increase the predictive value of spirometry. Peripheral blood mononuclear cells were isolated from 10 patients with CF and acute pulmonary exacerbations before and after therapy. RNA expression profiling revealed that 10 genes significantly changed with treatment when compared with matched non-CF and control subjects with stable CF to establish baseline transcript abundance. Peripheral blood mononuclear cell RNA transcripts were prospectively validated, using real-time polymerase chain reaction amplification, in an independent cohort of acutely ill patients with CF (n = 14). Patients who responded to therapy were analyzed using general estimating equations and multiple...Continue Reading

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Citations

Mar 7, 2009·American Journal of Respiratory and Critical Care Medicine·Felix Ratjen
Jul 23, 2013·Chest·Alborz Hakimi ShokiBradley S Quon
Feb 10, 2018·Annals of the American Thoracic Society·Milene T SaavedraJerry A Nick
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Mar 9, 2019·American Journal of Respiratory Cell and Molecular Biology·Xi ZhangHara Levy
Jan 5, 2021·Frontiers in Cardiovascular Medicine·Ahmad Aljohmani, Daniela Yildiz
Nov 1, 2009·Current Opinion in Pulmonary Medicine
Jul 1, 2009·Current Opinion in Endocrinology, Diabetes, and Obesity

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