Circulating Tumor Cells Enriched by the Depletion of Leukocytes with Bi-Antibodies in Non-Small Cell Lung Cancer: Potential Clinical Application

PloS One
Jian YinHongbing Shen

Abstract

It has been considered that the detection methods for circulating tumor cells (CTCs) based on epithelial cell adhesion molecule (EpCAM) underestimate the number of CTCs and may miss a metastatic subpopulation with cancer stem cell (CSC) properties. Therefore, we investigated EpCAM-positive and -negative CTCs in non-small cell lung cancer (NSCLC) patients at different stages, assessed the clinical value of these CTCs and explored their capacity in the following CSC model. CTCs were enriched by the depletion of leukocytes with bi-antibodies using a magnetic bead separation technique and then identified by the expression of EpCAM and cytokeratin 7 and 8 using multi-parameter flow cytometry. We determined the distribution of CTCs classified by the expression of EpCAM in 46 NSCLC patients with stages I to IV, assessed the diagnostic value of these CTCs by longitudinal monitoring in 4 index patients during adjuvant therapy and characterized the stemness of these CTCs by the expression of CXCR4 and CD133 in 10 patients. EpCAM-negative (E-) CTCs were detected to be significantly higher than EpCAM-positive (E+) CTCs in stage IV (p = 0.003). The patients with the percentage of E-CTCs more than 95% (r > 95%) were detected to be significan...Continue Reading

References

Oct 27, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·W Jeffrey AllardLeon W M M Terstappen
Jun 17, 2005·Cancer Research·Denis A SmirnovS Mark O'Hara
Sep 12, 2008·Nature Reviews. Cancer·Jane E Visvader, Geoffrey J Lindeman
Mar 25, 2009·Nature Reviews. Cancer·Don X NguyenJoan Massagué
Mar 23, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Matthew G KrebsFiona H Blackhall
Mar 26, 2011·Science·Christine L Chaffer, Robert A Weinberg
Sep 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yen-Tsung HuangDavid C Christiani
Dec 17, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Matthew G KrebsCaroline Dive
Mar 22, 2012·Journal of Translational Medicine·Alberto FusiUlrich Keilholz
Sep 28, 2012·Clinical Chemistry·Catherine Alix-Panabières, Klaus Pantel
Jun 8, 2013·Journal of the National Comprehensive Cancer Network : JNCCN·David S EttingerUNKNOWN National comprehensive cancer network
Sep 14, 2013·Science·Vicki PlaksZena Werb
Jul 25, 2014·Nature Reviews. Cancer·Zhao ChenKwok-Kin Wong
Aug 27, 2014·Nature Reviews. Cancer·Catherine Alix-Panabières, Klaus Pantel
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal

❮ Previous
Next ❯

Citations

Aug 30, 2019·Journal of Translational Medicine·Stephanie N ShishidoPeter Kuhn
Dec 28, 2018·Cancers·Marta Tellez-GabrielDominique Heymann
Mar 31, 2017·Molecular and Clinical Oncology·Yongping LiuChangsong Zhang
Sep 21, 2018·American Society of Clinical Oncology Educational Book·Xiaoliang WuPatrick C Ma
Jan 31, 2017·Annual Review of Pathology·J-A ThieleP Kuhn
Sep 29, 2020·Frontiers in Cell and Developmental Biology·Theresa D AhrensMette Ø Agerbæk
May 26, 2021·Technology in Cancer Research & Treatment·Helin WangShucai Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
FACS

Software Mentioned

FlowJo
Stata
SPSS ( Statistical Package for the Social Sciences )
ISET
CellSearch
SPSS

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Adhesion Molecules in AS

Cell adhesion molecules expressed on the vascular endothelium and circulating leukocytes in response to inflammatory stimuli are implicated in atherosclerosis. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.