Circulating tumor cells in different stages of colorectal cancer

Folia Histochemica Et Cytobiologica
Petra EliasovaVladimir Bobek

Abstract

Liquid biopsies are noninvasive tests using blood or body fluids to detect circulating tumor cells (CTCs) or the products of tumor cells, such as fragments of nucleic acids or proteins that are shed into biological fluids from primary tumor or its metastates. The analysis of published clinical studies provides coherent evidence that the presence of CTCs detected in peripheral blood is a strong prognostic factor in patients with colorectal carcinoma (CRC). The aim of the study was to implement size-based separation protocol of CTCs in CRC patients. Patients diagnosed with different stages of CRC (n = 98) were included in the study. All patients have been diagnosed for colorectal adenocarcinoma by pathology examination, 45 patients with colon carcinoma and 53 with rectosigmoid cancer. A size-based separation method (MetaCell®) for viable CTC enrichment from peripheral blood was used to assess the presence of CTCs by cytomorphological evaluation using vital fluorescence microscopy. Cytomorphological analysis revealed that 81 (83%) tested samples were CTC-positive and 17 (17%) were CTC-negative. We report a successful isolation of CTCs with proliferation potential in patients with CRC. The CTCs were cultured in vitro for further do...Continue Reading

Citations

Nov 30, 2019·Expert Review of Anticancer Therapy·Lahiri Kanth NanduriUlrich Bork
May 31, 2020·Cancers·Drahomír KolenčíkPeter Kuhn
Dec 6, 2017·Expert Review of Anticancer Therapy·Anthony LopezJaffer A Ajani
Jun 13, 2020·Frontiers in Oncology·Veronika VoronovaMarina Sekacheva
Apr 17, 2020·Micromachines·Jennifer QuinchiaJahir Orozco
Nov 11, 2020·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·Nigel P MurrayEghon Guzman
Feb 6, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Pamela PinzaniSilvia Galbiati
May 1, 2021·International Journal of Molecular Sciences·Donatella VerbanacKarmela Barišić
Sep 3, 2021·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·Nigel P MurraySocrates Aedo

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