Circulating tumor cells: potential markers of minimal residual disease in ovarian cancer? a study of the OVCAD consortium

Oncotarget
Eva ObermayrBurkhard Brandt

Abstract

In 75% of ovarian cancer patients the tumor mass is completely eradicated by established surgical and cytotoxic treatment; however, the majority of the tumors recur within 24 months. Here we investigated the role of circulating tumor cells (CTCs) indicating occult tumor load, which remains inaccessible by established diagnostics. Blood was taken at diagnosis (baseline samples, n = 102) and six months after completion of adjuvant first-line chemotherapy (follow-up samples; n = 78). CTCs were enriched by density gradient centrifugation. A multi-marker immunostaining was established and further complemented by FISH on CTCs and tumor/metastasis tissues using probes for stem-cell like fusion genes MECOM and HHLA1. CTCs were observed in 26.5% baseline and 7.7% follow-up blood samples at a mean number of 12.4 and 2.8 CTCs per ml blood, respectively. Baseline CTCs indicated a higher risk of death in R0 patients with complete gross resection (univariate: HR 2.158, 95% CI 1.111-4.191, p = 0.023; multivariate: HR 2.720, 95% CI 1.340-5.522, p = 0.006). At follow-up, the presence of CTCs was associated with response to primary treatment as assessed using RECIST criteria. Chromosomal gains at MECOM and HHLA1 loci suggest that the observed ce...Continue Reading

References

Jun 28, 1995·Journal of Immunological Methods·C GriwatzK S Zänker
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·Y Yarden, M X Sliwkowski
Apr 9, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T Tot
Dec 27, 2005·Gene·Nicola NormannoDavid S Salomon
Feb 23, 2007·Cancer Letters·Patrizia Paterlini-Brechot, Naoual Linda Benali
Jun 12, 2008·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Sabine Riethdorf, Klaus Pantel
Jan 1, 2009·Journal of the National Cancer Institute·Anieta M SieuwertsJohn A Foekens
Jan 13, 2009·Nature Cell Biology·Dorothea MaetzelOlivier Gires
Feb 2, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J FerlayE Steliarova-Foucher
May 8, 2010·Breast Cancer Research and Treatment·Katharina E EffenbergerBjorn Naume
Jul 2, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Natalia BednarzBurkhard Brandt
Aug 18, 2010·International Journal of Cancer. Journal International Du Cancer·Pauline WimbergerHeidi Schwarzenbach
Oct 5, 2010·Lancet·Gordon J S RustinUNKNOWN EORTC 55955 investigators
Jan 29, 2011·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Gordon John Sampson RustinUNKNOWN Gynecological Cancer Intergroup
May 27, 2011·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Bahriye AktasPauline Wimberger
Jan 24, 2012·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Stephan PolterauerJalid Sehouli
Jun 30, 2012·Neoplasia : an International Journal for Oncology Research·Arezou A GhazaniRalph Weissleder
Jan 11, 2013·Cell Adhesion & Migration·Teresa M Horm, Joyce A Schroeder
Jan 30, 2013·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Radoslav ChekerovJalid Sehouli
Mar 25, 2014·Gynecologic Oncology·Nuria Romero-LaordenIvan Diaz-Padilla
Jun 28, 2014·Gynecologic Oncology·Michael L PearlWen-Tien Chen
Jul 16, 2014·Cancer Cell·Sunila PradeepAnil K Sood
Oct 27, 2015·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Bradley R CorrCarol A Sartorius
Jun 7, 2016·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·C Van BerckelaerP A van Dam

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Citations

Sep 29, 2018·Cancers·Laurie G HudsonAngela Wandinger-Ness
Jul 22, 2018·Current Medicinal Chemistry·Jinfeng Zou, Edwin Wang
Aug 27, 2018·Virchows Archiv : an International Journal of Pathology·Ben Davidson
Jun 23, 2018·Clinical & Experimental Metastasis·John K ChanRobert L Coleman
Dec 18, 2020·Journal of Clinical Medicine·Masahiro TakakuraToshiyuki Sasagawa

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Methods Mentioned

BETA
density gradient centrifugation
chromosomal aberrations
blood draw

Software Mentioned

AdnaTest
OVCAD
Genepix
Integrachip
Nexus
SPSS

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