Abstract
The cancer secretome is a valuable reservoir of cancer biomarkers. Besides containing circulating tumor cells, extracellular vesicles, and proteins, it is also rich in circulating tumor DNA (ctDNA)-a subpopulation of cell free DNA. The most efficient technology to capture ctDNA is next generation sequencing (NGS). Indeed, this analysis enables the identification of both quantitative (e.g., mutant allelic fraction - MAF) and qualitative (e.g., the variant type) information. Strikingly, by calculating these data in relation to time, cytopathologists can decodify and graphically report the ctDNA "message", which may help to diagnose cancer, define treatment, and monitor disease evolution. In this paper, we report the most compelling evidence steadily accumulating on the successful application of NGS-based ctDNA analysis in cancer diagnosis, treatment decision, and monitoring of cancer progression. We also propose a mathematical model that calculates MAF evolution in relation to time.
References
Feb 15, 2013·Gut·Timothy Robert ChurchUNKNOWN PRESEPT Clinical Study Steering Committee, Investigators and Study Team
Jul 10, 2013·Nature Reviews. Clinical Oncology·Emily CrowleyAlberto Bardelli
Jun 25, 2015·Annals of Oncology : Official Journal of the European Society for Medical Oncology·Y-L WuY Zuo
Jul 30, 2016·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Karen L ReckampShirish Gadgeel
Dec 14, 2016·The New England Journal of Medicine·Tony S MokUNKNOWN AURA3 Investigators
Jan 29, 2017·Expert Review of Molecular Diagnostics·Umberto MalapelleCarlos Camps
Mar 3, 2017·Nature Reviews. Clinical Oncology·Giulia SiravegnaAlberto Bardelli
Aug 18, 2017·Science Translational Medicine·Jillian PhallenVictor E Velculescu
Jun 10, 2018·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Christian RolfoDavid R Gandara
Oct 27, 2018·Computational and Structural Biotechnology Journal·Maha Elazezy, Simon A Joosse
Aug 3, 2019·Human Genomics·Ming Chen, Hongyu Zhao
Aug 24, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kezhong ChenJun Wang
Sep 11, 2019·Nature Medicine·Aparna R ParikhRyan B Corcoran
Sep 19, 2019·Molecular Oncology·Sergio VillatoroMiguel A Molina-Vila
Oct 13, 2019·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Florian Scherer
Oct 15, 2019·Expert Review of Molecular Diagnostics·Alessandro RussoChristian Rolfo
Jan 29, 2020·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G SiravegnaA Bardelli
Feb 6, 2020·Oncotarget·Rabih SaidApostolia M Tsimberidou
Apr 30, 2020·Science·Anne Marie LennonNickolas Papadopoulos
May 5, 2020·Cancer·Philip C MackDavid R Gandara
May 20, 2020·Critical Reviews in Oncology/hematology·Christian RolfoUNKNOWN ISLB
Nov 15, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xinghao AiShun Lu
Jan 29, 2021·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M C LiuUNKNOWN CCGA Consortium
Feb 6, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Pamela PinzaniSilvia Galbiati