Circulating ultrasound-assisted extraction, countercurrent chromatography, and liquid chromatography for the simultaneous extraction, isolation, and analysis of the constituents of Uncaria tomentosa

Journal of Chromatography. a
Yuchi ZhangYuchun Li

Abstract

A hyphenated automated technique for the online extraction, isolation, analysis, and identification of natural organic compounds was established. Circulating ultrasound-assisted extraction (CUAE) was coupled with countercurrent chromatography (CCC), high performance liquid chromatography (HPLC), and a diode array detector (DAD). This approach was applied to the fractionation and purification of alkaloids from Uncaria tomentosa. A biphasic solvent system of chloroform-methanol-water (6:4:5, v:v:v) was used for the CUAE and CCC separation of compounds from 500 g of U. tomentosa. Two CUAE/CCC/HPLC/DAD modes were established. Either the upper aqueous phase or the lower organic phase of the solvent system could be used as the extraction solvent. The target compounds were extracted by CUAE, and the extract was pumped into a sample loop before being directly injected into the CCC column, or pre-purified using a flash chromatography column before injection. The target compounds were eluted using either the organic or aqueous phase of the solvent system and the fractions were monitored using a UV detector. The target fractions were collected by a sample loop via a six-port valve, and analyzed by HPLC/DAD for purity and structural identi...Continue Reading

References

Dec 7, 2007·Phytochemical Analysis : PCA·Nadezhda AngelovaRob Verpoorte
Dec 15, 2012·Chemistry, an Asian Journal·Hailong ZhangRui Wang
Dec 10, 2013·Ultrasonics Sonochemistry·Simon BothJochen Strube
Feb 14, 2014·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Lei-Lei ChenMin Li
Mar 19, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Tin-Yun HoChing-Liang Hsieh
Aug 1, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Houcai HuangLiang Feng

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Citations

Apr 2, 2016·Journal of Chromatography. a·Man WangDavid Da Yong Chen
Apr 8, 2016·Neurochemical Research·Meizhu ZhengYuchi Zhang
May 10, 2016·Journal of Chromatography. a·Man WangDavid Da Yong Chen
May 1, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying ZhouYuanjiang Pan
Dec 14, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shu-Xiao ChenXiao-Jie Gong
Jul 7, 2020·Experimental Gerontology·Lívia G CastilhosDaniela B R Leal

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