Circulation of Crimean-Congo Hemorrhagic Fever Virus in the former Yugoslav Republic of Macedonia revealed by screening of cattle sera using a novel enzyme-linked immunosorbent assay

PLoS Neglected Tropical Diseases
Marc MertensMartin H Groschup

Abstract

There are only few assays available for the detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)-specific antibodies in animals, and data about diagnostic sensitivity and specificity are incompletely documented for most of these tests. This is unfortunate since CCHFV antibodies in animals can be used as indicator for virus circulation in a geographic area and therewith potential risk of human exposure. This paper therefore reports on a novel ELISA for the detection of CCHFV-specific antibodies in cattle and on its application for testing ruminant sera from the Former Yugoslav Republic of Macedonia. A highly sensitive and specific ELISA was developed to detect CCHFV-specific IgG antibodies in cattle. The assay was validated by using 503 negative serum samples from a country where CCHFV has never been detected until now, and by using 54 positive serum samples. The positive sera were verified by using two commercially available assays (for testing human serum) which we have adapted for use in animals. The sensitivity of the novel ELISA was 98% and its specificity 99%. The presence of Hyalomma ticks was demonstrated in the Former Yugoslav Republic of Macedonia and depending on the region antibody prevalence rates up to 80% we...Continue Reading

References

Feb 1, 1989·The American Journal of Tropical Medicine and Hygiene·T M LoganJ R Moulton
Dec 17, 1998·Tropical Medicine & International Health : TM & IH·A AltafS Fisher-Hoch
Nov 20, 2004·Antiviral Research·Chris A Whitehouse
Mar 24, 2006·The Lancet Infectious Diseases·Onder Ergönül
May 17, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·O ErgonulB Dokuzoguz
Jul 7, 2007·Veterinary Parasitology·A IcaA Inci
Jan 17, 2009·Medical Microbiology and Immunology·Marc MertensRainer G Ulrich
Feb 26, 2009·Emerging Infectious Diseases·Turabi GunesZiynet Cinar
Sep 22, 2010·Vector Borne and Zoonotic Diseases·Sadegh ChinikarMichele Bouloy
Jan 21, 2012·Emerging Infectious Diseases·Agustín Estrada-PeñaJosé A Oteo
Apr 25, 2012·Parasitology Research·Zdenek Hubálek, Ivo Rudolf
Mar 6, 2013·Antiviral Research·Marc MertensMartin H Groschup

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Citations

Dec 26, 2015·Experimental & Applied Acarology·Isolde SchusterMartin H Groschup
Jul 29, 2016·Vector Borne and Zoonotic Diseases·Marc MertensMartin H Groschup
Mar 28, 2017·Vector Borne and Zoonotic Diseases·Josipa KulešMangesh Bhide
Jun 13, 2017·Vector Borne and Zoonotic Diseases·Miriam A SasMartin H Groschup
Jan 8, 2016·PLoS Neglected Tropical Diseases·Jessica R SpenglerPierre E Rollin
Mar 3, 2020·PLoS Neglected Tropical Diseases·Lía Monsalve-ArteagaMoncef Belhassen-García
Mar 23, 2019·BMJ Global Health·Laura T Mazzola, Cassandra Kelly-Cirino
Jul 19, 2017·The American Journal of Tropical Medicine and Hygiene·Ousmane MaigaMartin H Groschup
Mar 23, 2021·Current Tropical Medicine Reports·Paul A KuehnertKeersten M Ricks
Aug 8, 2021·Microorganisms·Julia HartlaubMartin H Groschup
Aug 15, 2021·Veterinary Medicine and Science·Médiha Khamassi KhbouMohamed Gharbi

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Methods Mentioned

BETA
ELISA
ELISAs

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