CIRP Induces Neutrophil Reverse Transendothelial Migration in Sepsis

Shock
Hui JinPing Wang

Abstract

Extracellular cold-inducible RNA-binding protein (CIRP) exaggerates inflammation in sepsis. Neutrophil reverse transendothelial migration (rTEM) allows neutrophils to migrate from tissues into the circulation. The phenotype of neutrophils after reverse migration is CD54CXCR1. We hypothesize that CIRP induces neutrophil rTEM in sepsis. Sepsis was induced in male C57BL/6 mice by cecal ligation and puncture (CLP), and at 5, 10, or 20 h after CLP the frequencies of reversely migrated (RM) neutrophils were assessed in the blood by flow cytometry. As 20 h of CLP showed highest increase in the frequency of RM neutrophils, we further assessed RM neutrophils in the blood of WT and CIRP mice at this time point. The effect of CIRP on neutrophil rTEM was determined by injecting mice with recombinant mouse CIRP (rmCIRP) intratracheally (i.t.) and assessed the frequencies of RM neutrophils. The expression of neutrophil elastase (NE) and junctional adhesion molecule-C (JAM-C) in the lungs was measured by Western blot. The mean frequency of RM neutrophils in sham mice was 0.4%, whereas the frequencies were significantly increased to 1%, 3%, and 7% at 5, 10, and 20 h of CLP, respectively. The mean frequency of RM neutrophils in the blood of CIR...Continue Reading

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Citations

Jan 16, 2019·Journal of Leukocyte Biology·Monowar AzizPing Wang
Apr 16, 2019·Shock·Rachel M Russo, Hasan B Alam
Jan 1, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kehong ChenPing Wang
Mar 3, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Brandon K Ashley, Umer Hassan
Apr 2, 2021·Frontiers in Immunology·Jingjing Ji, Jie Fan
Dec 26, 2021·Journal of Cellular and Molecular Medicine·Peng ZhongHe Huang

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