ciRS-7 Promotes the Proliferation and Migration of Papillary Thyroid Cancer by Negatively Regulating the miR-7/Epidermal Growth Factor Receptor Axis.

BioMed Research International
Jun-Ya HanKui-Sheng Chen

Abstract

The incidence of papillary thyroid cancer (PTC) is increasing, and traditional diagnostic methods are unsatisfactory. Therefore, identifying novel prognostic markers is very important. ciRS-7 has been found to play an important role in many cancers, but its role in PTC has not been reported. This study was performed to evaluate the biological role and mechanism of ciRS-7 in PTC. Material and Methods. The expression of ciRS-7 in PTC tissues and the matched adjacent tissues was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The PTC cell lines (TPC-1 and BCPAP) were used to evaluate the role of ciRS-7. ciRS-7-siRNA and overexpression plasmid were constructed and transfected into PTC cells. A CCK-8 assay and colony formation assay were performed to explore the effects of ciRS-7 on cell proliferation. Annexin V/PI staining and FACS detection were used to detect cell apoptosis. Wound healing assay was performed to detect cell migration. A transwell assay was conducted to explore the effects of ciRS-7 on invasion and migration. Western blotting was performed to evaluate protein expression. The luciferase reporter system was used to determine the underlying mechanism of miR-7. ciRS-7 was highly ex...Continue Reading

References

May 23, 2001·Current Biology : CB·S Bogdan, C Klämbt
Jun 23, 2015·Journal of Receptor and Signal Transduction Research·Yu SunHua-Fu Zhou
Nov 26, 2015·Molecular Diagnosis & Therapy·Zahra Nozhat, Mehdi Hedayati
May 11, 2016·Current Medicinal Chemistry·Weiyan ChengXiaojian Zhang
Sep 12, 2016·Journal of Cancer Research and Clinical Oncology·Liangliang XuMingqing Xu
Nov 16, 2016·European Thyroid Journal·Neslihan KurtulmusMete Duren
Nov 24, 2016·Cancer Metastasis Reviews·Christine L ChafferRobert A Weinberg
Feb 9, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wenhao WengAjay Goel
Aug 10, 2017·Methods in Molecular Biology·Zhixiang Wang
Jan 14, 2018·Cancer Letters·Bing Chen, Shenglin Huang
Jul 31, 2018·Expert Review of Endocrinology & Metabolism·Poupak FallahiSilvia Martina Ferrari
Jul 31, 2018·Expert Review of Endocrinology & Metabolism·Amanda Doubleday, Rebecca S Sippel
Aug 10, 2018·Experimental & Molecular Medicine·Jian SongJian-Yuan Jiang
Aug 16, 2019·Cancer Management and Research·Rizeng LiXiaoping Yang

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
transfections
PCR
Assay
transfection
flow cytometry

Software Mentioned

TargetScan
SPSS
PicTar
miRanda
Genema
miRBase

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis