cis-Acting determinants of heterochromatin formation on Drosophila melanogaster chromosome four

Molecular and Cellular Biology
Fang-Lin SunS C Elgin

Abstract

The heterochromatic domains of Drosophila melanogaster (pericentric heterochromatin, telomeres, and the fourth chromosome) are characterized by histone hypoacetylation, high levels of histone H3 methylated on lysine 9 (H3-mK9), and association with heterochromatin protein 1 (HP1). While the specific interaction of HP1 with both H3-mK9 and histone methyltransferases suggests a mechanism for the maintenance of heterochromatin, it leaves open the question of how heterochromatin formation is targeted to specific domains. Expression characteristics of reporter transgenes inserted at different sites in the fourth chromosome define a minimum of three euchromatic and three heterochromatic domains, interspersed. Here we searched for cis-acting DNA sequence determinants that specify heterochromatic domains. Genetic screens for a switch in phenotype demonstrate that local deletions or duplications of 5 to 80 kb of DNA flanking a transposon reporter can lead to the loss or acquisition of variegation, pointing to short-range cis-acting determinants for silencing. This silencing is dependent on HP1. A switch in transgene expression correlates with a switch in chromatin structure, judged by nuclease accessibility. Mapping data implicate the 1...Continue Reading

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Citations

Mar 1, 2006·Chromosoma·Kathryn L HuisingaSarah C R Elgin
Jul 6, 2006·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Nicole C Riddle, Sarah C R Elgin
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May 21, 2005·Nucleic Acids Research·Filomena De LuciaFang-Lin Sun
Sep 19, 2007·Genes & Development·Brent Brower-TolandHaifan Lin
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Nicole C RiddleSarah C R Elgin
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Oct 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Lixia PanFang-Lin Sun
Feb 26, 2008·Current Opinion in Genetics & Development·Tobias Straub, Peter B Becker

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