cis-Acting elements and trans-acting proteins in the transcriptional inhibition of gonadotropin-releasing hormone gene by human chorionic gonadotropin in immortalized hypothalamic GT1-7 neurons.

The Journal of Biological Chemistry
Z M Lei, C V Rao

Abstract

We investigated the cis-acting elements and trans-acting proteins required for the transcriptional inhibition of the gonadotropin-releasing hormone (GnRH) gene by human chorionic gonadotropin (hCG) in GT1-7 neurons. Transient transfection of GT1-7 neurons with the 5'-flanking region of the rat GnRH gene-luciferase fusion constructs revealed that a 53-base pair (bp) sequence between -126 and -73 bp is required for the hCG inhibition. Nuclear extracts from GT1-7 neurons contained 110- and 95-kDa proteins that formed two complexes with the 53-bp sequence. These proteins are not related to Fos, cAMP response element-binding protein, Oct-1, or progesterone receptors, and hCG treatment selectively increased the 95-kDa protein. DNase I footprinting with GT1-7 cell nuclear extracts protected the -99 to -79-bp region, which contained a so-called imperfect AP-1 site (-99 to -94 bp) and two AT-rich palindromic sequences (-91 to -87 bp and -85 to -81 bp). The mutagenesis of the AT-rich regions, but not the AP-1 site, resulted in a loss of DNA binding of the 95-kDa protein and the inhibitory effect of hCG. In summary, our results are consistent with hCG inducing a 95-kDa trans-acting protein, which binds to -91- to -81-bp AT-rich sequences ...Continue Reading

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T Hai, T Curran
Nov 3, 1989·Cell·M Levine, J L Manley
Aug 25, 1988·Nature·G Gill, M Ptashne
Jan 1, 1986·Journal of Neuroscience Research·D W Pfaff
Jan 1, 1966·Endocrinology·M A DavidL Martini
Jan 1, 1983·Endocrine Reviews·S P Kalra, P S Kalra
Jan 1, 1984·Cell and Tissue Research·I MerchenthalerB Flerkó
Mar 1, 1994·Molecular and Cellular Endocrinology·J M Bruder, M E Wierman
Jan 1, 1993·Annual Review of Cell Biology·B M Herschbach, A D Johnson
Mar 1, 1996·Molecular and Cellular Endocrinology·J K KepaM E Wierman

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Citations

Oct 17, 2002·Frontiers in Neuroendocrinology·J Peter H Burbach
May 24, 2003·Frontiers in Neuroendocrinology·Suzanne M MoenterCraig S Nunemaker
May 4, 2002·Molecular and Cellular Endocrinology·C V Rao, Z M Lei
Aug 29, 1998·Molecular and Cellular Endocrinology·S B NelsonP L Mellon
Jul 25, 2000·Molecular Human Reproduction·D R CorleyC V Rao
Oct 31, 2008·The FEBS Journal·Vien H Y LeeBilly K C Chow
Jul 10, 1999·The Journal of Biological Chemistry·B M FadelT Quertermous

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