cis-Acting packaging motifs of porcine adenovirus type 3

Virus Research
Li Xing, Suresh Kumar Tikoo

Abstract

The cis-acting packaging domain is required for selective encapsidation of adenovirus DNA into preformed empty capsids late in the viral life cycle. Earlier, it was demonstrated that the cis-acting packaging domain of porcine adenovirus type (PAdV)-3 is located between nucleotide position (nt) 212 and 531 at the left end genome which contains six AT/GC rich motifs. Removal of packaging domain from left end to the right end of the genome produced a viable mutant virus suggesting that the identified cis-acting packaging domain represents the DNA sequences required for selective packaging of PAdV-3 DNA, whose position and orientation appear to be flexible. Here, by constructing and analyzing a panel of virus mutants carrying deletions or linker scanning mutations in AT/GC rich sequences, we examined the significance of the continuous A/T or G/C sequences individually in the viral packaging process. In contrast to consensus bipartite structure (5'-TTTGN8CG-3') described for most of packaging motifs of human adenovirus type 5 (HAdV-5), the packaging motifs I, II, III, and IV of PAdV-3 displayed a tripartite structure in which the continuous A/T nucleotides were flanked by G/C-rich sequences. Mutations in both continuous A/T nucleoti...Continue Reading

References

Jul 1, 1975·Journal of Comparative Pathology·J B DerbyshireA P Collins
Jan 1, 1967·Archiv für die gesamte Virusforschung·M C ClarkeJ B Derbyshire
Feb 23, 2000·Journal of Virology·W Zhang, M J Imperiale
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Mar 7, 2002·Virology·Y Zhou, S K Tikoo
Apr 15, 2003·Journal of Virology·Philomena Ostapchuk, Patrick Hearing
May 14, 2003·Journal of Virology·Ece ErturkPatrick Hearing
Sep 5, 2003·Virology·Alexander ZakhartchoukSuresh Kumar Tikoo

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